Ducibella T, Rangarajan S, Anderson E
Department of Obstetrics/Gynecology, Tufts University, School of Medicine, Boston, Massachusetts 02111.
Dev Biol. 1988 Dec;130(2):789-92. doi: 10.1016/0012-1606(88)90368-5.
The basis for the incompetence of the cortical reaction in germinal vesicle stage (GV) mouse oocytes was studied by evaluating cortical granules (CGs) and vesicles in GV and mature oocyte cortices. Dark and light CGs had a similar mean distance of 0.4-0.6 micron from the plasma membrane for GV and mature cortices. The cortex of mature oocytes had a large population of membrane-bounded, 0.1-1.0 micron (diameter) vesicles. More than three times as many vesicles were observed in the CG domains of mature oocytes as were observed in GV oocytes. This lack of cortical vesicles (with their potential to store calcium) and not CG depth may account for cortical reaction incompetence in GV oocytes.
通过评估生发泡期（GV）小鼠卵母细胞和成熟卵母细胞皮质中的皮质颗粒（CGs）和囊泡，研究了GV期小鼠卵母细胞皮质反应无能的基础。对于GV期和成熟皮质，深色和浅色CGs距质膜的平均距离相似，为0.4 - 0.6微米。成熟卵母细胞的皮质有大量直径为0.1 - 1.0微米的膜结合囊泡。在成熟卵母细胞的CG区域观察到的囊泡数量是GV期卵母细胞中观察到的囊泡数量的三倍多。这种皮质囊泡（具有储存钙的潜力）的缺乏而非CG深度可能是GV期卵母细胞皮质反应无能的原因。
