School of Chemical and Materials Engineering, National University of Sciences & Technology, H-12, 44000 Islamabad, Pakistan.
School of Materials Sciences & Engineering, Nanyang Technological University, Nanyang Avenue, Singapore 639798, Singapore.
Sensors (Basel). 2020 Jan 23;20(3):646. doi: 10.3390/s20030646.
Interleukin 6 (IL-6), being a major component of homeostasis, immunomodulation, and hematopoiesis, manifests multiple pathological conditions when upregulated in response to viral, microbial, carcinogenic, or autoimmune stimuli. High fidelity immunosensors offer real-time monitoring of IL-6 and facilitate early prognosis of life-threatening diseases. Different approaches to augment robustness and enhance overall performance of biosensors have been demonstrated over the past few years. Electrochemical- and fluorescence-based detection methods with integrated electronics have been subjects of intensive research due to their ability to offer a better signal-to-noise ratio, high specificity, ultra-sensitivity, and wide dynamic range. In this review, the pleiotropic role of IL-6 and its clinical significance is discussed in detail, followed by detection schemes devised so far for their quantitative analysis. A critical review on underlying signal amplification strategies and performance of electrochemical and optical biosensors is presented. In conclusion, we discuss the reliability and feasibility of the proposed detection technologies for commercial applications.
白细胞介素 6（IL-6）作为内稳定、免疫调节和造血的主要组成部分，在响应病毒、微生物、致癌或自身免疫刺激而上调时表现出多种病理状况。高保真免疫传感器可实时监测 IL-6 并有助于危及生命的疾病的早期预后。过去几年已经证明了多种增强生物传感器稳健性和整体性能的方法。基于电化学和荧光的检测方法与集成电子设备由于能够提供更好的信噪比、高特异性、超高灵敏度和宽动态范围而成为研究的热点。在这篇综述中，详细讨论了 IL-6 的多效作用及其临床意义，以及迄今为止为其定量分析而设计的检测方案。对电化学和光学生物传感器的基本信号放大策略和性能进行了批判性评价。最后，我们讨论了所提出的检测技术用于商业应用的可靠性和可行性。
