Department of Surgery, Hanyang University Guri Hospital, Guri, Republic of Korea.
Department of Surgery, Dongguk University Medical Center, Gyeongju, Republic of Korea.
Pathol Res Pract. 2020 Mar;216(3):152821. doi: 10.1016/j.prp.2020.152821. Epub 2020 Jan 10.
This study aimed to investigate the clinicopathological and prognostic impact of B-cell linker (BLNK) protein expression in colorectal cancer (CRC) as its function in CRC remains unexplored. We performed immunohistochemical staining for BLNK using tissue microarrays of 418 consecutive CRC samples; of these 10 were excluded due to inappropriate staining. The expression intensity and staining level was scored as 0-3 and 0-4, respectively, based on the percentage of positive cells. The immunoreactivity score (IRS) was calculated by multiplying these two scores. BLNK expression was observed in 222 patients (54.4 %). Lymph node metastasis (p = 0.031), right colon cancer (p = 0.026), mucinous adenocarcinoma (p < 0.001), and perineural invasion (p = 0.049) were more frequently observed in the IRS 4-12 group than in the IRS 0-3 group. At the same cutoff point, the 5-year recurrence-free survival rate of the patients with stage III was significantly lower than that observed in IRS 4-12 group (74.8 % ± 4.2 % vs. 54.2 % ± 8.5 %, p = 0.003). Multivariate analysis revealed IRS 4-12 to be an independent risk factor for recurrence (Hazard ratio 2.346, 95 % confidence interval 1.348-4.085, p = 0.003). In conclusion, overexpression of BLNK protein is an independent risk factor for CRC recurrence.
本研究旨在探讨 B 细胞连接蛋白（BLNK）在结直肠癌（CRC）中的表达的临床病理和预后影响，因为其在 CRC 中的功能尚不清楚。我们使用 418 例连续 CRC 样本的组织微阵列对 BLNK 进行了免疫组织化学染色；其中 10 例由于染色不当而被排除在外。根据阳性细胞的百分比，将表达强度和染色水平分别评为 0-3 和 0-4。通过将这两个分数相乘计算免疫反应评分（IRS）。在 222 名患者（54.4%）中观察到 BLNK 表达。IRS 4-12 组的淋巴结转移（p = 0.031）、右结肠癌（p = 0.026）、黏液性腺癌（p < 0.001）和神经周围侵犯（p = 0.049）的发生率高于 IRS 0-3 组。在相同的截止点，III 期患者的 5 年无复发生存率明显低于 IRS 4-12 组（74.8%±4.2%比 54.2%±8.5%，p=0.003）。多因素分析显示 IRS 4-12 是复发的独立危险因素（危险比 2.346，95%置信区间 1.348-4.085，p=0.003）。总之，BLNK 蛋白的过表达是 CRC 复发的独立危险因素。
