Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Int J Dev Neurosci. 2020 Apr;80(2):96-105. doi: 10.1002/jdn.10008. Epub 2020 Feb 6.
Hypoxia-Ischemia (HI) is the most common cause of death and disability in human infants. The use of opiate in pregnant women affects their children. The aim of this study was to evaluate the effect of morphine consumption during pregnancy and lactation on vulnerability to neonatal HI in rats.
Female Wistar rats were randomly assigned into two groups: Group 1-Rats that did not receive any treatment during pregnancy and lactation and Group 2-Rats that received morphine during pregnancy and lactation. After delivery, male offspring were divided into four groups including: (a) SHAM, (b) SHAM/Morphine (SHAM/MO), (c) HI, (d) HI/Morphine (HI/MO). Seven days after HI induction, neurobehavioral tests were performed, and then, brain tissue was taken from the skull to measure cerebral edema, infarct volume, inflammatory factors, oxidative stress, and brain-derived neurotrophic factor (BDNF).
Total antioxidant capacity (TAC) and BDNF levels in the HI/MO group were significantly lower than HI and SHAM groups. TNF-α, C-reactive protein and total oxidant capacity levels in the HI/MO group were significantly higher than HI and SHAM groups. Cerebral edema and infarct volume in the HI/MO group were significantly higher than the HI group.
Based on the results, morphine consumption during pregnancy and lactation enhanced the deleterious effects of HI injury in pups.
缺氧缺血（HI）是导致人类婴儿死亡和残疾的最常见原因。孕妇使用阿片类药物会影响其子女。本研究旨在评估妊娠和哺乳期使用吗啡对大鼠新生 HI 易感性的影响。
雌性 Wistar 大鼠随机分为两组：第 1 组-妊娠和哺乳期未接受任何治疗的大鼠；第 2 组-妊娠和哺乳期接受吗啡的大鼠。分娩后，雄性幼鼠分为四组：（a）SHAM，（b）SHAM/吗啡（SHAM/MO），（c）HI，（d）HI/吗啡（HI/MO）。HI 诱导后 7 天，进行神经行为学测试，然后从颅骨中取出脑组织，测量脑水肿、梗死体积、炎症因子、氧化应激和脑源性神经营养因子（BDNF）。
HI/MO 组的总抗氧化能力（TAC）和 BDNF 水平明显低于 HI 和 SHAM 组。HI/MO 组的 TNF-α、C 反应蛋白和总氧化剂水平明显高于 HI 和 SHAM 组。HI/MO 组的脑水肿和梗死体积明显高于 HI 组。
根据结果，妊娠和哺乳期使用吗啡增强了 HI 损伤对幼仔的有害影响。
