MOE Key Laboratory of Bioinformatics, Bioinformatics Division, BNRIST, Beijing 100084, China; School of Life Sciences, Center for Synthetic & System Biology, Tsinghua University, Beijing 100084, China.
MOE Key Laboratory of Bioinformatics, Bioinformatics Division, BNRIST, Beijing 100084, China; School of Life Sciences, Center for Synthetic & System Biology, Tsinghua University, Beijing 100084, China; Department of Automation, Tsinghua University, Beijing 100084, China.
Genomics. 2020 May;112(3):2418-2425. doi: 10.1016/j.ygeno.2020.01.014. Epub 2020 Jan 22.
Alternative splicing contributes to the diversity of gene products by producing multiple transcript variants from one gene. Previous studies have revealed highly variable splicing patterns in single cells, but there is still a controversy in the understanding of the simultaneous expression of multiple transcript variants. Here we show that the dominance of a single transcript variant is a common phenomenon in single cells. We analyzed several single-cell RNA sequencing datasets and observed consistent results. Our results demonstrate that single cells tend to express one major transcript variant of a gene, and the diversity of transcript variants in cell populations mainly results from the heterogeneity of splicing pattern in single cells.
可变剪接通过从一个基因产生多个转录变体来增加基因产物的多样性。之前的研究已经揭示了单细胞中高度可变的剪接模式，但对于多个转录变体的同时表达的理解仍存在争议。在这里，我们表明，单个转录变体的优势是单细胞中的普遍现象。我们分析了几个单细胞 RNA 测序数据集，并观察到了一致的结果。我们的结果表明，单细胞倾向于表达一个基因的主要转录变体，细胞群体中转录变体的多样性主要源于单细胞中剪接模式的异质性。
