Scheemaeker S, Meyer E, Schoeman J P, Defauw P, Duchateau L, Daminet S
Department of Small Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Department of Pharmacology, Biochemistry and Toxicology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Vet J. 2020 Jan;255:105423. doi: 10.1016/j.tvjl.2019.105423. Epub 2019 Dec 30.
Neutrophil gelatinase-associated lipocalin (NGAL) is used as an early biomarker of renal injury in people. In dogs, increases in urinary NGAL (uNGAL) precede increases in serum creatinine (sCr) in experimental and clinical evaluations of acute kidney injury (AKI) and chronic kidney disease. This study compared uNGAL in two subsets of dogs with AKI and their respective controls. One set included dogs with snake-envenomation at risk for or presenting with International Renal Interest Society (IRIS) grade I AKI; the other group included dogs with AKI, where renal injury was the result of various causes, and IRIS grade was ≥II. Additionally, this study evaluated haemoglobin (Hb) interference during NGAL analysis in Hb spiked urine and plasma from healthy dogs. In both AKI groups, uNGAL was significantly higher than in matched healthy control dogs (P<0.01). Moreover, uNGAL was significantly higher in dogs with IRIS grade ≥II AKI than in dogs at risk of IRIS grade I AKI (P=0.04). In dogs at risk of IRIS grade I AKI, there were no significant differences in uNGAL and uNGAL/uCr between dogs bitten by cytotoxic or neurotoxic snakes (P=0.44). Additionally, Hb did not interfere with the canine NGAL immunoassay. In conclusion, this study confirms the value of uNGAL as a biomarker for early renal damage: uNGAL was significantly increased in dogs with snake-envenomation at risk for or presenting with IRIS grade I AKI, which could be left undiagnosed if evaluated with the traditional renal biomarker sCr. In addition, Hb did not interfere with NGAL measurement in dogs.
中性粒细胞明胶酶相关脂质运载蛋白（NGAL）被用作人类肾损伤的早期生物标志物。在犬类中，在急性肾损伤（AKI）和慢性肾病的实验及临床评估中，尿NGAL（uNGAL）升高先于血清肌酐（sCr）升高。本研究比较了两组患有AKI的犬及其各自对照组的uNGAL。一组包括有发生国际肾脏兴趣协会（IRIS）I级AKI风险或已出现该情况的被蛇咬伤犬；另一组包括AKI由各种原因导致且IRIS分级≥II级的犬。此外，本研究评估了健康犬的血红蛋白（Hb）加标尿液和血浆在NGAL分析过程中的干扰情况。在两个AKI组中，uNGAL均显著高于配对的健康对照犬（P<0.01）。此外，IRIS分级≥II级AKI的犬的uNGAL显著高于有IRIS I级AKI风险的犬（P=0.04）。在有IRIS I级AKI风险的犬中，被细胞毒性或神经毒性蛇咬伤的犬之间uNGAL和uNGAL/uCr无显著差异（P=0.44）。此外，Hb不干扰犬NGAL免疫测定。总之，本研究证实了uNGAL作为早期肾损伤生物标志物的价值：有发生IRIS I级AKI风险或已出现该情况的被蛇咬伤犬的uNGAL显著升高，若用传统肾脏生物标志物sCr评估可能无法诊断。此外，Hb不干扰犬的NGAL测量。
