人类肝脏DNA中O4-乙基胸腺嘧啶的检测
Detection of O4-ethylthymine in human liver DNA.
作者信息
Huh N, Satoh M S, Shiga J, Kuroki T
机构信息
Department of Cancer Cell Research, University of Tokyo, Japan.
出版信息
IARC Sci Publ. 1988(89):292-5.
O4-Ethyl-2'-deoxythymidine (O4-EtdThy) in human liver DNA was quantified, in order to monitor possible human exposure to ethylating agents, using a highly sensitive immunological detection method. In 30 of 33 cases analysed, O4-EtdThy was detected at above the detection limit (i.e., 3 x 10(-8) O4-EtdThy/2'-deoxy-thymidine[dThy]), indicating actual chronic exposure to ethylating agents. The mean content of O4-EtdThy in 19 cases of malignant tumours was significantly higher than that in 11 nonmalignant cases (p less than 0.05).
为监测人体可能接触的乙基化剂，采用高灵敏度免疫检测方法对人肝DNA中的O4-乙基-2'-脱氧胸苷（O4-EtdThy）进行定量分析。在33例被分析的样本中，有30例检测到O4-EtdThy高于检测限（即3×10⁻⁸ O4-EtdThy/2'-脱氧胸苷[dThy]），表明实际存在慢性乙基化剂暴露。19例恶性肿瘤患者的O4-EtdThy平均含量显著高于11例非恶性疾病患者（p<0.05）。
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