Hemminki K, Savela K, Linkola E, Hesso A
Institute of Occupational Health, Helsinki, Finland.
IARC Sci Publ. 1988(89):306-9.
A method has been developed for detecting 7-alkylguanines which is based on derivatization of hydroxyl and primary amino groups with 3H-acetic anhydride. The derivatization procedure is devised for 7-methylguanine, with examination of reaction kinetics. The method is applied to the detection of the isomeric styrene oxide-guanine adducts; styrene-7,8-oxide is the active metabolite of styrene. The procedure can be used as a postlabelling method for the simultaneous detection of several adducts after selective depurination of 7-alkylguanines from DNA.
已开发出一种基于用³H-乙酸酐对羟基和伯氨基进行衍生化来检测7-烷基鸟嘌呤的方法。衍生化程序是针对7-甲基鸟嘌呤设计的，并对反应动力学进行了研究。该方法用于检测同分异构的氧化苯乙烯-鸟嘌呤加合物；苯乙烯-7,8-氧化物是苯乙烯的活性代谢物。该程序可用作一种后标记方法，用于在从DNA中选择性脱嘌呤后同时检测几种加合物。
