Hemminki K, Vodicka P
Center for Nutrition and Toxicology, Karolinska Institute, Novum, Huddinge, Sweden.
Toxicol Lett. 1995 May;77(1-3):153-61. doi: 10.1016/0378-4274(95)03285-1.
Styrene oxide, a metabolite of styrene, reacts with many centres in nucleosides but in DNA N-7-, N2- and O6-guanine are the main sites. A 32P-postlabelling method was developed for the detection of O6-styrene oxide DNA adducts from white blood cells. The method involved use of nuclease P1 and magnet transfer. The O6 adducts were detected at a fmol range with about 10% labelling efficiency. In lamination workers the O6 adducts, adjusted for adduct recovery, were detected at a level of 5 adducts/10(8) nucleotides.
氧化苯乙烯是苯乙烯的一种代谢产物，它能与核苷中的多个位点发生反应，但在DNA中，鸟嘌呤的N-7、N2和O6位是主要反应位点。已开发出一种32P后标记法，用于检测白细胞中的O6-氧化苯乙烯DNA加合物。该方法涉及使用核酸酶P1和磁转移。可在飞摩尔范围内检测到O6加合物，标记效率约为10%。在层压工人中，经加合物回收率调整后，检测到的O6加合物水平为5个加合物/10(8)个核苷酸。
