Watson W P, Smith R J, Huckle K R, Wright A S
Shell Research Ltd, Sittingbourne Research Centre, Kent, UK.
IARC Sci Publ. 1988(89):384-8.
In order to obtain information on the genotoxic metabolism of carcinogens in human skin in vivo, model in-vitro systems have been developed to mimic in-vivo metabolism qualitatively. Direct labelling (3H and 14C) and 32P-postlabelling analyses of benzo[alpha]pyrene (BP)-DNA adducts in human skin explants, CD1 mouse explants and CD1 mouse skin in vivo have thus allowed comparisons of the genotoxic metabolism of BP in mouse and human skin.
为了获取致癌物在人体皮肤内的遗传毒性代谢信息，已开发出体外模型系统以定性模拟体内代谢。因此，通过对人皮肤外植体、CD1小鼠外植体和CD1小鼠体内皮肤中苯并[a]芘（BP）-DNA加合物进行直接标记（3H和14C）和32P后标记分析，得以比较BP在小鼠和人皮肤中的遗传毒性代谢。
