皮内接种二价 EV71-CA16 灭活疫苗在恒河猴中诱导的综合保护免疫研究。
Study of integrated protective immunity induced in rhesus macaques by the intradermal administration of a bivalent EV71-CA16 inactivated vaccine.
机构信息
Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Kunming 650118, China.
Aimei Convac BioPharm (Jiangsu) Co., Ltd., Taizhou 225300, Jiangsu, China.
出版信息
Vaccine. 2020 Feb 18;38(8):2034-2044. doi: 10.1016/j.vaccine.2019.12.057. Epub 2020 Jan 22.
Enterovirus type 71 (EV71) and coxsackievirus A 16 (CA16) are recognized as the major pathogens responsible for human hand-foot-mouth disease. To develop a bivalent EV71-CA16 vaccine, rhesus macaques immunized with two doses of this vaccine via the intradermal route were challenged with EV71 or CA16, and their clinical symptoms, viral shedding, neutralizing antibodies, IFN-γ-specific ELISpots, and tissue viral load were examined longitudinally. Specific immunity against EV71 and CA16 was observed in the macaques, which exhibited controlled proliferation of the EV71 and CA16 viruses and upregulated expression of immune-related genes compared with the controls. Furthermore, broad protection against EV71 and CA16 challenge without immunopathological effects was observed in all the immunized macaques. These studies suggest that the bivalent EV71-CA16 inactivated vaccine was effective against wild-type EV71 or CA16 viral challenge in rhesus macaques.
肠道病毒 71 型（EV71）和柯萨奇病毒 A16 型（CA16）被认为是导致人类手足口病的主要病原体。为了开发 EV71-CA16 二价疫苗，通过皮内途径给恒河猴免疫两剂该疫苗，然后用 EV71 或 CA16 进行攻毒，纵向检测其临床症状、病毒脱落、中和抗体、IFN-γ 特异性 ELISpots 和组织病毒载量。恒河猴对 EV71 和 CA16 表现出特异性免疫，与对照组相比，EV71 和 CA16 病毒的增殖受到控制，免疫相关基因的表达上调。此外，所有免疫的恒河猴均观察到对 EV71 和 CA16 攻毒的广泛保护而没有免疫病理作用。这些研究表明，该 EV71-CA16 二价灭活疫苗对野生型 EV71 或 CA16 病毒攻毒在恒河猴中是有效的。
Zotero 插件