研究碱木质素的化学修饰，以应用于纤维素酶吸附剂。
Study on the chemical modification of alkali lignin towards for cellulase adsorbent application.
机构信息
State Key Laboratory of Pulp and Papermaking, School of Light Industry and Engineering, South China University of Technology, China.
State Key Laboratory of Pulp and Papermaking, School of Light Industry and Engineering, South China University of Technology, China.
出版信息
Int J Biol Macromol. 2020 Apr 15;149:794-800. doi: 10.1016/j.ijbiomac.2020.01.229. Epub 2020 Jan 23.
The research of cost-efficient lignin-based adsorbents is a practical strategy for the recovery of cellulase. In this study, alkali lignin was modified to increase the phenolic hydroxyl (Ph-OH) content for cellulase adsorption applications. After phenolation, compared with the lignin reference, the maximum adsorption cellulase capacity of lignoresorcinol (LigR) and lignopyrogallol (LigP) was improved from 76.5 mg/g to 842.1 mg/g and 911.4 mg/g, respectively. The enzyme activity of the adsorbed cellulase on LigR was higher than that on LigP, which could migrate to the fresh substrates during enzymatic hydrolysis. The adsorbed cellulase could be easily recovered from two lignin-based adsorbents by adjusting pH. The distinct cellulase adsorption behavior of two lignin-based adsorbents was closely related to the high Ph-OH contents and low S/G ratio in phenolated lignin samples characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Heteronuclear Single Quantum Coherence-Nuclear Magnetic Resonance (HSQC-NMR).
研究经济高效的木质素基吸附剂是回收纤维素酶的一种实用策略。在这项研究中，对碱木质素进行了修饰，以增加酚羟基（Ph-OH）含量，用于纤维素酶吸附应用。经过苯氧基化后，与木质素参比物相比，木质素连苯三酚（LigR）和木质素焦儿茶酚（LigP）的最大吸附纤维素酶容量分别从 76.5 mg/g 提高到 842.1 mg/g 和 911.4 mg/g。吸附在 LigR 上的酶活性高于吸附在 LigP 上的酶活性，因为在酶水解过程中它可以迁移到新鲜的底物上。通过调节 pH 值，可以从两种木质素基吸附剂中轻松回收吸附的纤维素酶。两种木质素基吸附剂的明显纤维素酶吸附行为与傅里叶变换红外光谱（FTIR）和异核单量子相干-核磁共振（HSQC-NMR）表征的苯氧基化木质素样品中高 Ph-OH 含量和低 S/G 比密切相关。
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