Department of Dentistry, Federal University of Rio Grande Norte, Natal, RN, Brazil.
Department of Dentistry, Federal University of Rio Grande Norte, Natal, RN, Brazil.
Arch Oral Biol. 2020 Mar;111:104664. doi: 10.1016/j.archoralbio.2020.104664. Epub 2020 Jan 16.
Spondias mombin has been used in folk medicine to treat inflammation in the oral cavity. This study aimed to evaluate the antioxidant and anti-inflammatory activity of S. mombin extract in an oral mucositis experimental model.
Male hamsters were orally pre-treated with hydroethanolic extract of S. mombin leaves (HESM) (50, 100, or 200 mg/kg) for ten days. Cheek pouch samples were subjected to macroscopic, histopathological and immunohistochemical analysis (Cox-2, iNOS, NF-kB p50 NLS and MMP-2). IL-1β and TNF-α levels were analyzed by ELISA immunoassay, and Superoxide dismutase estimative (SOD), glutathione (GSH) and malondialdehyde (MDA) levels were submitted to spectroscopy analysis.
The group treated with HESM at a dose of 200 mg/kg showed the best healing effect, showing no evidence of ulceration in the macroscopic analysis (p < 0.05). Histopathological analysis showed re-epithelialization, discrete mononuclear inflammatory infiltrate and absence of hemorrhage and edema score of 1 (1-1) (p < 0.05), as well as a large amount of collagen fibers and a lower immunoexpression of Cox-2, iNOS, NF-kB p50 NLS and MMP-2. Decrease in SOD (p < 0.05), MDA (p<0.001), IL-1β (p < 0.05), and TNF-α levels (p < 0.001), with an increase in GSH (p < 0.01) levels.
HESM (200 mg/kg) reduced oxidative stress and inflammation in the 5-fluorouracil-induced oral mucositis in hamsters.
黄皮树在民间医学中用于治疗口腔炎症。本研究旨在评估黄皮树叶水乙醇提取物（HESM）在口腔黏膜炎实验模型中的抗氧化和抗炎活性。
雄性金黄地鼠经口预先用 HESM（50、100 或 200mg/kg）处理 10 天。颊囊样本进行宏观、组织病理学和免疫组织化学分析（Cox-2、iNOS、NF-kB p50 NLS 和 MMP-2）。通过 ELISA 免疫分析法分析 IL-1β 和 TNF-α 水平，并用光谱分析法分析超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和丙二醛（MDA）水平。
200mg/kg HESM 组的愈合效果最佳，宏观分析未见溃疡证据（p<0.05）。组织病理学分析显示再上皮化、离散的单核炎症浸润，无出血和水肿评分 1（1-1）（p<0.05），以及大量胶原纤维和 Cox-2、iNOS、NF-kB p50 NLS 和 MMP-2 的免疫表达减少。SOD（p<0.05）、MDA（p<0.001）、IL-1β（p<0.05）和 TNF-α 水平降低（p<0.001），GSH 水平升高（p<0.01）。
HESM（200mg/kg）降低了氟尿嘧啶诱导的金黄地鼠口腔黏膜炎的氧化应激和炎症。
