Guo Jiajie, Yoshida Kaya, Ikegame Mika, Okamura Hirohiko
Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata, Kitaku, Okayama, 770-8525, Japan; Department of Endodontics, School of Stomatology, China Medical University, Nanjing North Street 117, Shenyang, 110002, China.
Department of Oral Healthcare Education, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8504, Japan.
J Oral Biosci. 2020 Mar;62(1):16-29. doi: 10.1016/j.job.2020.01.001. Epub 2020 Jan 23.
Bacteria exhibit multi-cellular social behavior, such as biofilm formation, virulence generation, bioluminescence, or sporulation, through cell-to-cell communication involving a quorum sensing (QS) system capable of sensing species density. Pseudomonas aeruginosa (P. aeruginosa) is a ubiquitous gram-negative opportunistic pathogen that is frequently isolated from immunocompromised patients. It is particularly detected in patients with severe periodontitis and persistent endodontic infections, forcing a rethink of the role of this opportunistic pathogen in oral lesions.
N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) is a pivotal QS molecule, which regulates numerous virulence genes in P. aeruginosa and exhibits broad biological modulation effects in mammalian cells. In this review, we highlight the diverse OdDHL-mediated apoptosis and immunomodulatory effects on host cells. The structural properties, signaling pathways, targeted genes and proteins, and intracellular metabolism of OdDHL are also discussed to clarify the interactions between P. aeruginosa and the host.
The purpose of this review is to identify a valid target for quenching OdDHL, which could potentially eliminate the pathogenic effect of P. aeruginosa.
细菌通过涉及能够感知种群密度的群体感应（QS）系统的细胞间通讯，表现出多细胞社会行为，如生物膜形成、毒力产生、生物发光或孢子形成。铜绿假单胞菌是一种普遍存在的革兰氏阴性机会致病菌，经常从免疫功能低下的患者中分离出来。它在重度牙周炎和持续性牙髓感染患者中尤为常见，这促使人们重新思考这种机会致病菌在口腔病变中的作用。
N-（3-氧代十二烷酰基）-L-高丝氨酸内酯（OdDHL）是一种关键的QS分子，它调节铜绿假单胞菌中的众多毒力基因，并在哺乳动物细胞中表现出广泛的生物调节作用。在本综述中，我们重点介绍了OdDHL介导的对宿主细胞的多种凋亡和免疫调节作用。还讨论了OdDHL的结构特性、信号通路、靶向基因和蛋白质以及细胞内代谢，以阐明铜绿假单胞菌与宿主之间的相互作用。
本综述的目的是确定一个有效的靶点来淬灭OdDHL，这可能潜在地消除铜绿假单胞菌的致病作用。
