Laboratory of Cell Signaling, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 770-8513, Japan.
Laboratory of Cell Signaling, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 770-8513, Japan.
Toxicol In Vitro. 2020 Jun;65:104782. doi: 10.1016/j.tiv.2020.104782. Epub 2020 Jan 23.
Triphenyltin (TPT), previously used as an agricultural fungicide and industrial antifoulant, is now considered an environmental pollutant. The effect of TPT on human health is concerning due to its presence as a contaminant in seafood. In this study, the changes in intracellular Zn concentration ([Zn]i) and cellular content of nonprotein thiols ([NPT]i) induced by triphenyltin chloride (TPTCH), were measured in rat thymic lymphocytes. This was studied by flow-cytometry using the fluorescent probes FluoZin-3-AM and 5-chloromethylfluorescein diacetate (5-CMF-DA). Incubation with TPTCH, at 0.1 μM or more (up to 3 μM), increased [Zn]i in a concentration-dependent manner. The TPTCH-induced elevation in [Zn]i was due to the increase in membrane Zn permeability and intracellular Zn release. Incubation with TPTCH at 0.3 μM significantly increased [NPT]i levels, whereas the addition of an intracellular Zn chelator had no effect on the same. TPT at higher concentrations (1 or 3 μM) reduced [NPT]i. TPT may disturb intracellular Zn signaling in lymphocytes that disturbs cellular functions.
三苯基锡（TPT）曾被用作农业杀菌剂和工业防污剂，现在被认为是一种环境污染物。由于其作为海鲜污染物的存在，TPT 对人类健康的影响令人担忧。在这项研究中，使用荧光探针 FluoZin-3-AM 和 5-氯甲基荧光素二乙酸酯（5-CMF-DA），通过流式细胞术测量了三苯基氯化锡（TPTCH）诱导的大鼠胸腺淋巴细胞细胞内锌浓度（[Zn]i）和非蛋白巯基细胞内含量（[NPT]i）的变化。当 TPTCH 的浓度为 0.1 μM 或更高（最高达 3 μM）时，[Zn]i 呈浓度依赖性增加。TPTCH 诱导的 [Zn]i 升高是由于膜锌通透性增加和细胞内锌释放增加所致。用 0.3 μM 的 TPTCH 孵育可显著增加[NPT]i 水平，而加入细胞内锌螯合剂则对其没有影响。较高浓度的 TPT（1 或 3 μM）降低了[NPT]i。TPT 可能会扰乱淋巴细胞内的细胞内锌信号转导，从而扰乱细胞功能。
