Departments of Bioengineering, Hacettepe University, Turkey.
Departments of Restorative Dentistry, Hacettepe University, Turkey.
J Photochem Photobiol B. 2020 Mar;204:111742. doi: 10.1016/j.jphotobiol.2019.111742. Epub 2019 Dec 12.
Human dental pulp stem cells (hDPSCs) are able to differentiate into dopaminergic neurons and help the maintenance of partially degenerated neurons, which makes them as an alternative cell source for treatment of Parkinsons' disease (PD) patients. Here, the effect of photobiomodulation with polychromatic light source in the near infrared (NIR) range (600-1200 nm) or low level 660 nm diode laser light on hDPSCs during dopaminergic induction was investigated. Real time RT-qPCR analysis indicated that expressions of brain derived neurotrophic factor (BDNF), glial cell line derived neurotropic factor (GNDF), matrix associated protein 2 (MAP2), nuclear receptor related 1 protein (NURR1) and dopamine transporter (DAT) were increased, especially in the first 7 days of dopaminergic induction when 660 nm laser light was applied with a total energy density of 1.6 J/cm. The activity of polychromatic light on hDPSCs depended on the differentiation media and protein type. BDNF, GDNF, NURR-1 and MAP2 expressions were increased in the presence of pre-induction factors, and decreased when the post-induction factors were added into the culture medium. In contrast with all these promising results, the dopaminergically induced hDPSCs did not show any functional characteristics of dopaminergic neurons and died after they were transferred to a new laminin coated culture plates. In conclusion, the expression of dopaminergic neuron protective protein mRNAs in hDPSCs was increased by photobiomodulation in defined conditions. However, the cells were not able to differentiate into functional dopaminergic neurons either in control or in photobiomodulated groups that are prone to cell death and exhibit immature dopaminergic neuron characteristics.
人牙髓间充质干细胞（hDPSCs）能够分化为多巴胺能神经元，并有助于维持部分退化神经元的功能，这使其成为治疗帕金森病（PD）患者的替代细胞来源。在这里，研究了近红外（NIR）范围内（600-1200nm）多色光源或低水平 660nm 二极管激光光对多巴胺能诱导期间 hDPSCs 的光生物调节作用。实时 RT-qPCR 分析表明，脑源性神经营养因子（BDNF）、胶质细胞源性神经营养因子（GNDF）、基质相关蛋白 2（MAP2）、核受体相关 1 蛋白（NURR1）和多巴胺转运蛋白（DAT）的表达增加，尤其是在应用 660nm 激光光并施加 1.6J/cm 总能量密度的多巴胺能诱导的前 7 天。多色光对 hDPSCs 的活性取决于分化培养基和蛋白类型。在存在预诱导因子的情况下，BDNF、GDNF、NURR-1 和 MAP2 的表达增加，而当将诱导后因子添加到培养基中时，其表达减少。与所有这些有希望的结果相反，多巴胺能诱导的 hDPSCs 没有显示出多巴胺能神经元的任何功能特征，并且在将其转移到新的层粘连蛋白包被的培养板后死亡。总之，在确定的条件下，光生物调节可增加 hDPSCs 中多巴胺能神经元保护蛋白 mRNA 的表达。然而，无论是在对照组还是在光生物调节组中，这些细胞都不能分化为具有功能的多巴胺能神经元，这两组细胞容易死亡并表现出不成熟的多巴胺能神经元特征。
