Qi Aiqin, Han Juan, Jia Fengxia, Liu Chao
Department of Neurology, Jinan City People's Hospital, Jinan 271100, P.R. China.
J BUON. 2019 Nov-Dec;24(6):2465-2474.
This study aimed to investigate the expressions of microRNA (miR)-3175 and miR-134 in gliomas and their effects on the proliferation and invasion of U251 glioma cells.
Tumor tissues of 42 patients with glioma and non-tumor tissues of 10 patients with severe craniocerebral injury were collected. These patients were diagnosed in Jinan City People's Hospital from March 2010 to April 2012. qRT-PCR was used to detect differences of miR-3175 and miR-134 expressions. Kaplan-Meier method was used to generate survival curves and Log-rank test to evaluate differences between miR-3175, miR-134 and 5-year survival of the patients. Western blot was used to detect levels of p-PI3K in PI3K signaling pathway.
Proliferative activity of the cells in miR-3175 mimic group and miR-control group at different time points was significantly better than that of the cells in miR-3175 inhibitor group (p<0.05). The proliferative activity of the cells in miR-134 mimic group and miR-control group at different time points was significantly worse than that of the cells in miR-134 inhibitor group (p<0.05). Apoptosis rate of the cells in miR-3175 mimic group and miR control group was significantly lower than that of the cells in miR-3175 inhibitor group (p<0.05). Apoptosis rates of the cells in miR-134 mimic group and miR-control group were significantly higher than that of the cells in miR-134 inhibitor group (p<0.05).
Downregulating expression of miR-3175 or facilitating expression of miR-134 could inhibit the proliferative and invasive activity of glioma cells and facilitate their apoptosis by inhibiting the activation of PI3K signaling pathway.
本研究旨在探讨微小RNA（miR）-3175和miR-134在胶质瘤中的表达及其对U251胶质瘤细胞增殖和侵袭的影响。
收集2010年3月至2012年4月在济南市人民医院确诊的42例胶质瘤患者的肿瘤组织和10例重度颅脑损伤患者的非肿瘤组织。采用qRT-PCR检测miR-3175和miR-134表达的差异。采用Kaplan-Meier法绘制生存曲线，并采用Log-rank检验评估miR-3175、miR-134与患者5年生存率之间的差异。采用蛋白质免疫印迹法检测PI3K信号通路中p-PI3K的水平。
miR-3175模拟物组和miR-对照组细胞在不同时间点的增殖活性明显优于miR-3175抑制剂组细胞（p<0.05）。miR-134模拟物组和miR-对照组细胞在不同时间点的增殖活性明显低于miR-134抑制剂组细胞（p<0.05）。miR-3175模拟物组和miR-对照组细胞的凋亡率明显低于miR-第3175抑制剂组细胞（p<0.05）。miR-134模拟物组和miR-对照组细胞的凋亡率明显高于miR-134抑制剂组细胞（p<0.05）。
下调miR-3175的表达或促进miR-134的表达可抑制胶质瘤细胞的增殖和侵袭活性，并通过抑制PI3K信号通路的激活促进其凋亡。
