De Bruyne G K, Bracke M E, Plessers L, Mareel M M
Department of Radiotherapy, University Hospital, Gent, Belgium.
Invasion Metastasis. 1988;8(5):253-65.
Interactions between invasive and noninvasive cells were studied via confronting cultures between mixed cell aggregates and embryonic chick heart fragments in vitro. The mixed aggregates were composed of MCF-7 and HBL-100 cells, both derived from human mammary epithelium. HBL-100 cells invade into the heart fragments when confronted as unmixed aggregates, while MCF-7 cells do not. Using mixed aggregates HBL-100 cells still invade into the heart tissue, but MCF-7 cells sort out to the periphery of the cultures and do not invade. Two mechanisms concerning the noninvasiveness of MCF-7 cells in vitro are discussed: the homotypic adhesion of the MCF-7 cell population due to the presence of numerous desmosomes, and the incapability of MCF-7 cells to migrate on extracellular laminin present in the embryonic chick heart and in the HBL-100 cell population.
通过体外混合细胞聚集体与鸡胚心脏片段的对峙培养，研究了侵袭性细胞与非侵袭性细胞之间的相互作用。混合聚集体由MCF-7和HBL-100细胞组成，二者均来源于人乳腺上皮。当以未混合的聚集体形式对峙时，HBL-100细胞会侵入心脏片段，而MCF-7细胞则不会。使用混合聚集体时，HBL-100细胞仍会侵入心脏组织，但MCF-7细胞会分选到培养物的周边且不发生侵入。文中讨论了关于MCF-7细胞在体外非侵袭性的两种机制：由于存在大量桥粒，MCF-7细胞群体的同型黏附；以及MCF-7细胞无法在鸡胚心脏和HBL-100细胞群体中存在的细胞外层粘连蛋白上迁移。
