Le Marer N, Bruyneel E
National Institute For Medical Research, London, U.K.
Anticancer Res. 1996 Sep-Oct;16(5A):2767-72.
Different in vitro invasion assays have been established to assess tumour cell invasiveness. We compared the invasiveness of previously described cell lines from human breast cancer that are derived from cancers in an early or late stage of malignancy, by three different in vitro systems. First, we evaluated the capacity of the cells to go through a Matrigel barrier in a Boyden chamber, and in a second assay their ability to migrate in a thick layer of collagen. These experiments showed that early stage cells were more motile than late stage cells. By a third assay, we tested the ability of cells to invade chick heart fragment tissue and found none of the cells to be invasive.
已经建立了不同的体外侵袭试验来评估肿瘤细胞的侵袭性。我们通过三种不同的体外系统，比较了先前描述的源自早期或晚期恶性肿瘤的人乳腺癌细胞系的侵袭性。首先，我们评估了细胞在博伊登小室中穿过基质胶屏障的能力，在第二项试验中评估了它们在厚层胶原蛋白中迁移的能力。这些实验表明，早期细胞比晚期细胞更具运动性。通过第三种试验，我们测试了细胞侵入鸡心脏碎片组织的能力，发现没有细胞具有侵袭性。
