Villegas Ana M, Stabler Lisa, Moore Robert J, Uzal Francisco A, Lacey Jake A, Hofacre Charles, Lee Margie, Ferguson-Noel Naola, Barber Rosetta, Rimet Claire-Sophie, Jerry Carmen, Kim Woo Kyun, Madison Barquiesha, França Monique
Poultry Diagnostic and Research Center, Department of Population Health (Villegas, Stabler, Hofacre, Lee, Ferguson-Noel, Barber, Rimet, Jerry, Madison, Franca) and Department of Poultry Science (Villegas, Kim), University of Georgia, Athens, GA; California Animal Health and Food Safety Laboratory System, University of California-Davis, San Bernardino, CA (Uzal); School of Science, RMIT University, Bundoora West Campus, Bundoora, VIC, Australia (Moore); Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA (Lee); Doherty Department at the Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, VIC, Australia (Lacey).
J Vet Diagn Invest. 2020 Mar;32(2):268-276. doi: 10.1177/1040638720901726. Epub 2020 Jan 26.
Focal duodenal necrosis (FDN) is an intestinal disease of egg-layer chickens characterized by multifocal necrosis of the duodenal loop and proximal jejunum. Affected flocks usually have decreased egg weights and drops in egg production. Previous studies have associated this condition with infection. We tried to reproduce FDN by experimental infection of egg-laying chickens using different -positive and -negative strains, and duodenal homogenate obtained from FDN lesions. Chickens challenged with and/or duodenal homogenate developed duodenitis after challenge. Gross lesions included mucosal erosions, hyperemia, mucosal hemorrhages, and watery intestinal content. Microscopic lesions included mild enterocyte degeneration and necrosis, and mild-to-moderate hemorrhage and lymphoplasmacytic and heterophilic infiltration of the lamina propria. Two -positive strains closely related to necrotic enteritis pathogenic strains, by genomic composition, were re-isolated from lesions. Necrosis of intestinal crypts was observed in chickens challenged with duodenal homogenate with or without coinfection. Characteristic microscopic FDN lesions with significant necrosis and loss of villus enterocytes were not reproduced.
局灶性十二指肠坏死（FDN）是蛋鸡的一种肠道疾病，其特征为十二指肠袢和空肠近端出现多灶性坏死。受影响的鸡群通常蛋重下降且产蛋量减少。先前的研究将这种病症与感染联系起来。我们试图通过使用不同的阳性和阴性菌株以及从FDN病变中获取的十二指肠匀浆对产蛋鸡进行实验性感染来复制FDN。用[具体菌株]和/或十二指肠匀浆攻击的鸡在攻击后出现了十二指肠炎症。大体病变包括黏膜糜烂、充血、黏膜出血以及肠内容物水样化。显微镜下病变包括轻度肠上皮细胞变性和坏死，以及固有层轻度至中度出血和淋巴细胞、浆细胞及嗜异性粒细胞浸润。从病变中重新分离出了两种在基因组组成上与坏死性肠炎致病菌株密切相关的阳性菌株。在有或没有[具体菌株]共同感染的情况下，用十二指肠匀浆攻击的鸡出现了肠隐窝坏死。但并未复制出具有显著坏死和绒毛肠上皮细胞丢失的特征性显微镜下FDN病变。
