Digestive division, Endoscopic center, People's Hospital of Ningxia Hui Autunomous Region, Yinchuan, China.
Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan, China.
Gastroenterol Hepatol. 2020 May;43(5):240-247. doi: 10.1016/j.gastrohep.2019.10.006. Epub 2020 Jan 23.
Gastric cancer (GC) has been become the second leading cause for cancer-associated death. This study aimed to investigate Orexin A levels and associated receptors in tumor tissues of GC patients.
Forty-six consecutive gastric cancer patients (GC, n=46) and 13 chronic atrophic gastritis patients (CAG, n=13) were recruited. Meanwhile, 18 health individuals visiting Medical Examination Department were involved as control (N group, n=18). ELISA was used to examine Orexin A concentration. Immunohistochemistry assay was used to examine OX1R and OX2R. HE staining was applied to evaluate inflammation. qRT-PCR was employed to detect OX1R, OX2R, prepro-Orexin mRNAs. Serum Helicobacter pylori (H. pylori) infection was measured.
Orexin A expression in GC patients was significantly up-regulated compared to N group and CAG group (p<0.05). Orexin A expression was increased in CAG group compared to N group (p<0.05). Gastric cancer tissues exhibited significantly obvious inflammation compared to N group and CAG group (p<0.05). OX1R and OX2R expressions were significantly down-regulated in GC group compared to N group and CAG group (p<0.05). OX1R and OX2R were lower significantly in GC group compared to CAG group (p<0.05). Prepro-Orexin was significantly depleted in tumor tissues of GC group compared to N group and CAG group (p<0.05). Orexin A expression was un-associated with gender, age and differential grades (p>0.05). CAG and GC patients demonstrated higher H. pylori infection rates.
Orexin A was associated with inflammation by interacting with OX1R/OX2R receptor and activating prepro-Orexin in tumor tissues of gastric cancer patients.
胃癌（GC）已成为癌症相关死亡的第二大主要原因。本研究旨在探讨 GC 患者肿瘤组织中食欲素 A 水平及其相关受体。
招募了 46 例连续 GC 患者（GC 组，n=46）和 13 例慢性萎缩性胃炎患者（CAG 组，n=13）。同时，纳入 18 名来体检科就诊的健康个体作为对照组（N 组，n=18）。采用 ELISA 检测食欲素 A 浓度，免疫组化法检测 OX1R 和 OX2R，HE 染色评估炎症，qRT-PCR 检测 OX1R、OX2R、前食欲素 mRNA。检测血清幽门螺杆菌（H. pylori）感染情况。
GC 患者食欲素 A 表达明显高于 N 组和 CAG 组（p<0.05）。CAG 组食欲素 A 表达高于 N 组（p<0.05）。GC 患者胃组织炎症明显高于 N 组和 CAG 组（p<0.05）。GC 组 OX1R 和 OX2R 表达明显低于 N 组和 CAG 组（p<0.05）。GC 组 OX1R 和 OX2R 明显低于 CAG 组（p<0.05）。GC 组肿瘤组织中前食欲素明显减少（p<0.05）。食欲素 A 表达与性别、年龄和分化程度无关（p>0.05）。CAG 和 GC 患者 H. pylori 感染率较高。
食欲素 A 通过与 OX1R/OX2R 受体相互作用并激活 GC 患者肿瘤组织中的前食欲素，与炎症有关。
