Oishi Ryoutarou, Mizuta Tatsumi, Sueyoshi Kenji, Endo Tatsuro, Hisamoto Hideaki
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka, Sakai, Osaka, 599-8531, Japan.
PRESTO, JST, Japan Science Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan.
Anal Sci. 2020 Feb 10;36(2):143-145. doi: 10.2116/analsci.19C013. Epub 2020 Jan 24.
Herein, we describe the development of a novel material, "enzyme-responsive fluorescent ionic liquid", which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALP-labeled antibodies.
在此，我们描述了一种新型材料“酶响应荧光离子液体”的开发，它能够高度灵敏地检测碱性磷酸酶（ALP）。我们使用这种新材料作为增塑剂制备了增塑聚氯乙烯（PVC）膜，并对水溶液中的ALP进行了定量。初步结果表明，PVC膜在膜与样品溶液的界面处对ALP作出响应，通过阴离子萃取来维持膜相中的电中性。所开发的PVC膜的灵敏度比传统膜高出约六倍，从而证明了对ALP的高灵敏检测。这些结果表明，所提出的膜通过使用ALP标记抗体在高灵敏蛋白质检测方面具有潜在的适用性。
