Esfahrood Zeinab Rezaei, Ardakani Mohammadreza Talebi, Shokri Mehdi, Shokri Maryam
Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Indian Soc Periodontol. 2020 Jan-Feb;24(1):15-19. doi: 10.4103/jisp.jisp_218_19. Epub 2020 Jan 2.
Platelet products play a fundamental role in the process of healing. The new generation of platelet-rich fibrin (PRF), namely advanced PRF (A-PRF), has different biological and mechanical properties compared to those of leukocyte-PRF (L-PRF). This study aimed to compare the effects of L-PRF and A-PRF on the viability and migration of human gingival fibroblasts (HGFs).
In this study, the effects of A-PRF and L-PRF on the viability and migration of HGFs after 24 and 48 h were evaluated using the methyl thiazolyl tetrazolium assay. The viability of the negative control culture medium was considered to be 100%. The mean optical density of the test groups was divided by that of the negative control group and reported as percentage. One-way ANOVA was applied to assess the effects of time and type of PRF on the viability and migration of HGFs. Pairwise comparisons were made using the Tukey's test.
At 24 h, cell viability in the L-PRF group was significantly higher than that in the A-PRF group ( < 0.05). However, no significant difference was noted between the two groups at 48 h. At 24 h, L-PRF caused significantly higher cell migration compared to the negative control group, whereas at 48 h, both A-PRF and L-PRF significantly increased cell migration compared to the control group.
Within the limitations of this study, L-PRF and A-PRF had significant effects on the viability and migration of HGFs. Further studies on these platelet concentrates are warranted.
血小板制品在愈合过程中发挥着重要作用。新一代富含血小板纤维蛋白（PRF），即高级PRF（A-PRF），与白细胞PRF（L-PRF）相比具有不同的生物学和力学特性。本研究旨在比较L-PRF和A-PRF对人牙龈成纤维细胞（HGFs）活力和迁移的影响。
在本研究中，使用甲基噻唑基四氮唑法评估A-PRF和L-PRF在24小时和48小时后对HGFs活力和迁移的影响。将阴性对照培养基的活力视为100%。将测试组的平均光密度除以阴性对照组的平均光密度，并以百分比形式报告。应用单因素方差分析评估PRF的时间和类型对HGFs活力和迁移的影响。使用Tukey检验进行两两比较。
在24小时时，L-PRF组的细胞活力显著高于A-PRF组（<0.05）。然而，在48小时时，两组之间未观察到显著差异。在24小时时，与阴性对照组相比，L-PRF引起的细胞迁移显著更高，而在48小时时，与对照组相比，A-PRF和L-PRF均显著增加细胞迁移。
在本研究的局限性内，L-PRF和A-PRF对HGFs的活力和迁移有显著影响。有必要对这些血小板浓缩物进行进一步研究。
