Barón Marta, Morales Victoria, Fuentes Mª Victoria, Linares María, Escribano Nuria, Ceballos Laura
Faculty of Health Sciences, IDIBO Research Group, Rey Juan Carlos University, Madrid, Spain.
Group of Chemical and Environmental Engineering, School of Experimental Sciences and Technology, Rey Juan Carlos University, Madrid, Spain.
Aust Endod J. 2020 Aug;46(2):217-225. doi: 10.1111/aej.12395. Epub 2020 Jan 26.
The aim of this study was to analyse changes in radicular dentine composition after different irrigation regimes using attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR). Dentine slices from human single-root teeth were immersed in: (i) 5.25% sodium hypochlorite (NaOCl) for 1, 5 and 20 min; (ii) 17% EDTA for 1 min; and (iii) 5.25% NaOCl for 20 min, and 17% EDTA and 5.25% NaOCl, both for 1 min. Carbonate/mineral, Amide I/mineral and Amide III/CH ratios were determined using ATR-FTIR before and after immersion in the irrigation solutions tested. Results showed that 5.25% NaOCl significantly decreased carbonate/mineral, Amide I/mineral and Amide III/CH ratios (P < 0.05). Application of 17% EDTA produced no change in carbonate/mineral ratio (P = 0.120), while an increase in Amide I/mineral in apical third (P = 0.002) and Amide III/CH (P < 0.001) was observed. The combination of NaOCl, EDTA and NaOCl increased carbonate/mineral ratio in coronal third (P = 0.037), and Amide I/mineral (P = 0.003) and Amide III/CH (P = 0.001) ratios. In conclusion, irrigation solutions tested significantly affected radicular dentine composition.
本研究旨在使用衰减全反射傅里叶变换红外光谱法（ATR-FTIR）分析不同冲洗方案后根管牙本质成分的变化。将人类单根牙的牙本质切片浸泡于：（i）5.25%次氯酸钠（NaOCl）中1、5和20分钟；（ii）17%乙二胺四乙酸（EDTA）中1分钟；（iii）5.25% NaOCl中20分钟，以及17% EDTA和5.25% NaOCl中各1分钟。在浸泡于测试冲洗液前后，使用ATR-FTIR测定碳酸盐/矿物质、酰胺I/矿物质和酰胺III/CH比值。结果显示，5.25% NaOCl显著降低了碳酸盐/矿物质、酰胺I/矿物质和酰胺III/CH比值（P < 0.05）。应用17% EDTA后，碳酸盐/矿物质比值无变化（P = 0.120），而根尖三分之一处的酰胺I/矿物质（P = 0.002）和酰胺III/CH（P < 0.001）有所增加。NaOCl、EDTA和NaOCl联合使用增加了冠部三分之一处的碳酸盐/矿物质比值（P = 0.037），以及酰胺I/矿物质（P = 0.003）和酰胺III/CH（P = 0.001）比值。总之，测试的冲洗液显著影响了根管牙本质成分。
