School of Physics and Electronic Information, Yunnan Normal University, Kunming, 650500, China.
Institute of Quality Standards and Testing Technology, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China.
Sci Rep. 2020 Nov 23;10(1):20411. doi: 10.1038/s41598-020-77350-1.
Deficiency of selenium (Se) will lead to malnutrition and decreased immune function of the body. There is a common phenomenon of Se deficiency in foods. In this study, different concentrations of sodium selenite (NaSeO) were applied to Moringa oleifera grownin soil. The purpose was to explore the feasibility of Se biofortification of M. oleifera root. The effect of exogenous Se on the accumulation of Se and cadmium (Cd) in the roots of M. oleifera was studied by inductively coupled plasma mass spectrometry, and the mechanism of exogenous Se on the accumulation of Se and Cd in the roots was studied by Fourier transform infrared spectroscopy (FTIR) combined with principal component analysis and partial least squares regression analysis. The results showed that NaSeO significantly affected the accumulation of Se and Cd in the roots (p < 0.05). The increase in Se was highest when NaSeO was around 4.0 mg/kg, which increased by 315% compared with the control. The decrease in Cd was the lowest when NaSeO was around 2.0 mg/kg, which decreased by 80% compared with the control. The results of FTIR analysis showed that NaSeO treatment changed the carboxylate, phosphate radical, hemicellulose and protein in roots of M. oleifera, while the increase of Se was related to hemicellulose, protein, polysaccharide and lignin, and the decrease of Cd was related to hemicellulose and protein. The results showed that exogenous Se increased the accumulation of Se and inhibited the absorption of Cd. Therefore, the roots of M. oleifera can be used in Se biofortified products.
硒（Se）缺乏会导致营养不良和身体免疫功能下降。食物中普遍存在硒缺乏的现象。本研究将不同浓度的亚硒酸钠（NaSeO）应用于生长在土壤中的辣木（Moringa oleifera），旨在探索辣木根部硒生物强化的可行性。通过电感耦合等离子体质谱法（ICP-MS）研究了外源 Se 对辣木根部 Se 和镉（Cd）积累的影响，通过傅里叶变换红外光谱（FTIR）结合主成分分析和偏最小二乘回归分析研究了外源 Se 对辣木根部 Se 和 Cd 积累的作用机制。结果表明，NaSeO 显著影响根部 Se 和 Cd 的积累（p<0.05）。当 NaSeO 约为 4.0 mg/kg 时，Se 的增加量最高，比对照增加了 315%。当 NaSeO 约为 2.0 mg/kg 时，Cd 的减少量最低，比对照减少了 80%。FTIR 分析结果表明，NaSeO 处理改变了辣木根部的羧酸盐、磷酸根、半纤维素和蛋白质，而 Se 的增加与半纤维素、蛋白质、多糖和木质素有关，Cd 的减少与半纤维素和蛋白质有关。结果表明，外源 Se 增加了 Se 的积累并抑制了 Cd 的吸收。因此，辣木根部可用于 Se 生物强化产品。
