Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
Luminescence. 2020 Aug;35(5):763-772. doi: 10.1002/bio.3782. Epub 2020 Jan 26.
The development of an analytical probe to monitor highly mutagenic picric acid (PA) carries enormous significance for the environment and for health. A novel, simple and rapid fluorescence analytical assay using sulfur-doped graphene quantum dots (SGQDs) was designed for the highly sensitive and selective detection of PA. SGQDs were synthesized via simple pyrolysis of 3-mercaptopropionic acid and citric acid and characterized using advanced analytical techniques. Fluorescence intensity (FI) of SGQDs was markedly quenched by addition of PA, attributed to the inner filter effect and dominating static quenching mechanism between the two, in addition to a significant colour change. The calibration curve of the proposed assay exhibited a favourable linearity between quenched FI and PA concentration over the 0.1-100 μΜ range with a lowest detection limit of 0.093 μΜ and a correlation coefficient of 0.9967. The analytical assay was investigated for detection of trace amounts of PA in pond and rain water samples and showed great potential for practical applications with both acceptable recovery (98.0-100.8%) and relative standard deviation (1.24-4.67%). Analytical performance of the assay in terms of its detection limit, linearity range, and recovery exhibited reasonable superiority over previously reported methods, thereby holding enormous promise as a simple, sensitive, and selective method for detection of PA.
开发一种用于监测高诱变的苦味酸（PA）的分析探针对于环境和健康具有巨大的意义。本文设计了一种新颖、简单和快速的荧光分析测定法，使用了硫掺杂石墨烯量子点（SGQDs），用于高灵敏度和选择性检测 PA。SGQDs 通过 3-巯基丙酸和柠檬酸的简单热解合成，并通过先进的分析技术进行了表征。PA 的加入明显猝灭了 SGQDs 的荧光强度（FI），归因于内滤效应和两种物质之间的静态猝灭机制占主导地位，同时还发生了明显的颜色变化。该方法的校准曲线在 0.1-100 μΜ 范围内显示出良好的线性关系，最低检测限为 0.093 μΜ，相关系数为 0.9967。该分析测定法用于检测池塘和雨水样品中的痕量 PA，具有很大的实际应用潜力，回收率（98.0-100.8%）和相对标准偏差（1.24-4.67%）均可以接受。与先前报道的方法相比，该测定法在检测限、线性范围和回收率方面的分析性能具有明显的优势，因此作为一种简单、灵敏和选择性检测 PA 的方法具有巨大的应用前景。
