Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
Food Chem. 2020 Jul 1;317:126457. doi: 10.1016/j.foodchem.2020.126457. Epub 2020 Feb 20.
In this work, a novel, selective and sensitive fluorescent probe (sulfur doped graphene quantum dots, SGQDs) was designed for real-time detection of quercetin in red wine samples. SGQDs were synthesized by pyrolyzing citric acid (CA) and 3-Mercaptopropionic acid (MPA) and characterized through advanced techniques. It was observed that fluorescence intensity of SGQDs could be substantially quenched by the addition of quercetin through inner filter effect (IFE) mechanism. Additionally, a visual color change (colorless to light yellow) was also noticed after addition of quercetin into a solution of SGQDs. The change in SGQDs fluorescence intensity with varying quercetin content revealed good linearity in the 0-50.0 μM range with regression coefficient of 0.9943 and a lowest detection limit of 0.006 μg/mL. To authenticate the real-time application of SGQDs as a potential fluorescent probe, red wine samples having different quercetin concentrations were used for quantitative analysis, after the optimization of several analytical parameters.
在这项工作中，设计了一种新型的、选择性和灵敏的荧光探针（硫掺杂石墨烯量子点，SGQDs），用于实时检测红酒样品中的槲皮素。SGQDs 通过热解柠檬酸（CA）和 3-巯基丙酸（MPA）合成，并通过先进的技术进行了表征。观察到通过内滤效应（IFE）机制，SGQDs 的荧光强度可以通过添加槲皮素而大大猝灭。此外，在向 SGQDs 溶液中加入槲皮素后，还观察到溶液颜色发生明显变化（无色变为浅黄色）。随着槲皮素含量的变化，SGQDs 荧光强度的变化显示出在 0-50.0 μM 范围内具有良好的线性关系，回归系数为 0.9943，最低检测限为 0.006 μg/mL。为了验证 SGQDs 作为潜在荧光探针的实时应用，在优化了几个分析参数后，使用具有不同槲皮素浓度的红酒样品进行了定量分析。
