Otani T, Minami Y, Marunaka T, Zhang R, Xie M Y
Biological Research Laboratory, Taiho Pharmaceutical Co., Ltd., Tokushima, Japan.
J Antibiot (Tokyo). 1988 Nov;41(11):1580-5. doi: 10.7164/antibiotics.41.1580.
A new macromolecular antibiotic C-1027 was obtained from the broth filtrate of Streptomyces globisporus C-1027 by precipitation with ammonium sulfate, DEAE-cellulose column chromatography and gel filtration chromatography on a Sephadex G-75 column. This antibiotic, prepared as a white powder, is an acidic polypeptide having an isoelectric point of pH 3.5-3.7 and a molecular weight of 15,000 as determined by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography. The acid hydrolysate of the purified antibiotic C-1027 contained no methionine or tryptophan. From the physico-chemical data, it may be considered to possess a very labile non-protein chromophore.
通过硫酸铵沉淀、DEAE-纤维素柱色谱以及在Sephadex G-75柱上的凝胶过滤色谱，从球形链霉菌C-1027的发酵滤液中获得了一种新的大分子抗生素C-1027。这种抗生素制成白色粉末状，是一种酸性多肽，通过SDS-聚丙烯酰胺凝胶电泳和凝胶过滤色谱测定，其等电点为pH 3.5 - 3.7，分子量为15,000。纯化后的抗生素C-1027的酸水解产物中不含蛋氨酸或色氨酸。根据物理化学数据，可认为它具有一个非常不稳定的非蛋白质发色团。
