Nelson R A, Pope J A, Pandey R C, McDaniel L E, Schaffner C P, Beveridge R L, Hoops P H, Jordan F
Waksman Institute of Microbiology, Rutgers State University of New Jersey, Piscataway 08855-0759.
J Antibiot (Tokyo). 1988 Nov;41(11):1659-67. doi: 10.7164/antibiotics.41.1659.
The biosynthesis of crisamicin A, a novel dimeric isochromanequinone antibiotic from Micromonospora purpureochromogenes subsp. halotolerans has been investigated by [1-13C] and [2-13C] labeled acetate precursor feeding experiments. Analysis of the proton noise decoupled and off resonance 13C NMR spectra of 13C enriched and unenriched crisamicin A and their acetate derivatives indicated the biosynthesis via the polyketide pathway, as expected. Further analysis of the enriched spectra allowed the complete assignment of the carbon signals. Of particular interest was the establishment of the linkage between the two monomeric halves of the molecule and determination of the location of the phenolic hydroxyls.
通过[1-¹³C]和[2-¹³C]标记的乙酸前体喂养实验，对来自耐盐紫色小单孢菌亚种的一种新型二聚异苯并二氢吡喃醌抗生素——crisamicin A的生物合成进行了研究。对¹³C富集和未富集的crisamicin A及其乙酸衍生物的质子噪声去耦和¹³C异核单量子相干谱的分析表明，正如预期的那样，其生物合成是通过聚酮途径进行的。对富集谱的进一步分析使得碳信号得以完全归属。特别令人感兴趣的是确定分子两个单体部分之间的连接以及酚羟基的位置。
