The College of Life Sciences , Northwest University , Xi'an 710069 , China.
Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology , Northwest University , Xi'an 710069 , China.
J Agric Food Chem. 2020 Feb 19;68(7):2174-2182. doi: 10.1021/acs.jafc.9b07075. Epub 2020 Feb 5.
Goat milk oligosaccharides are complex carbohydrates with a variety of biological functions. Free oligosaccharides from goat milk show more similarity to human milk than cow milk. At present, changes in goat milk glycoconjugates at different parities remain poorly studied. Herein, we qualitatively and quantitatively compared the goat milk glycoprotein /-glycome at different parities using a stable isotope labeling followed by electrospray ionization mass spectrometry and online hydrophilic interaction chromatography. -Glycans were mainly fucosylated and nonfucosylated nonsialylated, and both fucosylation and sialylation gradually increased with parity, amounting (at the third parity) to 1.25 times and 3.3 times those of the first parity, respectively. -Glycans were mostly nonfucosylated and nonsialylated, and sialylation increased with increasing parity, and Neu5Ac-sialylated was up to 9 times higher in the third parity than in the first parity, whereas Neu5Gc-sialylated was 5.5 times higher. This study provides a reference for exploring an alternative milk source closest to human milk and for the development of humanized formula milk.
羊奶低聚糖是具有多种生物学功能的复杂碳水化合物。游离的羊奶低聚糖比牛奶更类似于人乳。目前，不同胎次羊奶糖缀合物的变化仍研究甚少。本研究采用稳定同位素标记结合电喷雾电离质谱和在线亲水作用色谱，定性和定量比较了不同胎次羊奶糖蛋白/糖组。-糖链主要为岩藻糖基化和非岩藻糖基化的非唾液酸化，岩藻糖基化和唾液酸化都随着胎次的增加而逐渐增加，分别达到（第三胎次）第一胎次的 1.25 倍和 3.3 倍。-糖链主要是非岩藻糖基化和非唾液酸化，随着胎次的增加而增加，唾液酸化增加，第三胎次 Neu5Ac 唾液酸化比第一胎次高 9 倍，而 Neu5Gc 唾液酸化高 5.5 倍。本研究为探索最接近人乳的替代奶源提供了参考，也为人乳化配方奶粉的开发提供了参考。
