Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China.
The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Carbohydr Polym. 2023 Sep 1;315:121004. doi: 10.1016/j.carbpol.2023.121004. Epub 2023 May 11.
O-glycome is one of the important components of glycoconjugates in human milk which is speculated to provide protective features similar to those observed in free oligosaccharides. The effects of maternal secretor status on free oligosaccharides and N-glycome in milk have been well researched and documented. Currently, milk O-glycome of secretors (Se+) and nonsecretors (Se-) was investigated through reductive β-elimination combined with porous graphitized carbon-liquid chromatography-electrospray ionization-tandem mass spectrometry. A total of 70 presumptive O-glycan structures were identified, of which 25 O-glycans (including 14 sulfated O-glycans) were reported for the first time. Notably, 23 O-glycans exhibited significant differences between Se+ and Se- samples (p < 0.05). Compared to Se- group, the O-glycans of the Se+ group was two times more abundant in the total glycosylation, sialylation, fucosylation, and sulfation (p < 0.01). In conclusion, approximately one-third of the milk O-glycosylation was influenced by maternal FUT2-related secretor status. Our data will lay a foundation for the study of O-glycans structure-function relationship.
人乳中的糖缀合物含有重要的 O-聚糖成分，据推测其具有类似于游离低聚糖的保护特性。母体分泌状态对母乳中游离低聚糖和 N-聚糖的影响已经得到了充分的研究和记录。目前，通过还原β-消除结合多孔石墨化碳-液相色谱-电喷雾电离-串联质谱法研究了分泌型（Se+）和非分泌型（Se-）人乳的 O-聚糖。共鉴定出 70 种假定的 O-聚糖结构，其中 25 种 O-聚糖（包括 14 种硫酸化 O-聚糖）为首次报道。值得注意的是，23 种 O-聚糖在 Se+和 Se-样本之间存在显著差异（p<0.05）。与 Se-组相比，Se+组的 O-聚糖在总糖基化、唾液酸化、岩藻糖化和硫酸化程度上分别增加了两倍（p<0.01）。总之，约三分之一的乳 O-糖基化受母体 FUT2 相关分泌状态的影响。我们的数据将为 O-聚糖结构-功能关系的研究奠定基础。
