CAS Key Laboratory of Separation Sciences for Analytical Chemistry , Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Zhongshan Road 457 , Dalian 116023 , China.
University of Chinese Academy of Sciences , Yuquan Road 19 , Beijing 100049 , China.
J Agric Food Chem. 2020 Feb 19;68(7):2263-2275. doi: 10.1021/acs.jafc.9b05568. Epub 2020 Feb 5.
The growth conditions and age of are vital for determining the quality of the ginseng plant. However, the considerable difference in price according to the cultivation method and period of leads to its adulteration in the trade market. We herein focused on ginseng peptides and the possibility of these peptides to be used as biomarker(s) for discrimination of . We applied an ultraperformance liquid chromatography-high resolution mass spectrometry-based peptidomics approach to characterize ginseng peptides and discover novel peptide biomarkers for authentication of mountain-cultivated ginseng (MCG). We identified 52 high-confidence peptides and screened 20 characteristic peptides differentially expressed between MCG and cultivated ginseng (CG). Intriguingly, 6 differential peptides were expressed significantly in MCG and originated from dehydrins that accumulated during cold or drought conditions. In addition, 14 other differential peptides that were significantly expressed in CG derived from ginseng major protein, an essential protein for nitrogen storage. These biological associations confirmed the reliability and credibility of the differential peptides. Additionally, we determined several robust peptide biomarkers for discrimination of MCG through a precise selection process. These findings demonstrate the potential of peptide biomarkers for identification and quality control of in addition to ginsenoside analysis.
人参的生长条件和年龄对其质量至关重要。然而，由于种植方法和周期的不同，人参价格差异相当大，导致其在贸易市场上存在掺假现象。本文主要关注人参肽，并探讨这些肽是否可作为区分人参的生物标志物。我们采用基于超高效液相色谱-高分辨质谱的肽组学方法来表征人参肽，并发现了用于鉴别野山参的新型肽生物标志物。我们共鉴定出 52 种高可信度肽，并筛选出 20 种在野山参与栽培人参之间差异表达的特征肽。有趣的是，有 6 种差异表达的肽来源于在低温或干旱条件下积累的脱水蛋白，在野山参中表达显著。此外，在 CG 中表达显著的 14 种其他差异表达的肽来自人参主要蛋白，这是一种用于氮储存的必需蛋白。这些生物学关联证实了差异肽的可靠性和可信度。此外，我们还通过精确的选择过程确定了几个用于区分野山参的稳健肽生物标志物。这些发现表明，除了人参皂苷分析之外，肽生物标志物还具有鉴定和控制人参质量的潜力。
