Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America.
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California, United States of America.
PLoS Biol. 2020 Jan 27;18(1):e3000567. doi: 10.1371/journal.pbio.3000567. eCollection 2020 Jan.
Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.
细胞和组织层面的过程通常需要数天或数周的时间才能发生，但很少有成像方法能够捕捉到如此长的时间尺度。在这里，我们描述了一种简单、非侵入性的方法 Bellymount，用于在亚细胞分辨率下对果蝇腹部进行纵向成像。Bellymounted 动物保持活体和完整，因此同一个体可以进行连续成像，从而获得多日过程的生动时间序列。这一特性为在其天然环境中对果蝇内部器官进行纵向研究打开了大门。利用 Bellymount 的功能，我们在单个动物中追踪肠道干细胞谱系和肠道微生物定植，揭示了以前可用方法无法检测到的时空动态。
