Akter Tasmina, Lindegaard Mikkel, Pedersen Karl, Strube Mikael L, Ronco Troels, Dalsgaard Inger
National Veterinary Institute, Technical University of Denmark, Anker Engelunds Vej 1, DK-2800, Kongens Lyngby, Denmark.
J Aquat Anim Health. 2020 Mar;32(1):21-27. doi: 10.1002/aah.10093. Epub 2020 Jan 27.
The genetic diversity of Vibrio anguillarum pJM1-like plasmids was investigated. Plasmids were isolated from 18 V. anguillarum serovar O1 strains collected from different geographic locations and fish species. The plasmids were sequenced and compared with the complete sequence of the published virulence plasmid pJM1. All 18 strains contained pJM1-like plasmids with approximately 65 kbp and all plasmids encoded the virulence genes responsible for the anguibactin iron sequestering system. The plasmids were highly conserved but minor differences were observed in some genes. A single nucleotide polymorphisms (SNPs) analysis showed 0-11 nucleotide variations between each of the 18 plasmids and the pJM1 plasmid. Compared with the sequence of pJM1, nonsynonymous SNPs were identified in fatC, angR, angL, pJM1_p19, and angE. In particular, a mutation found in 15 out of 18 sequenced plasmids in angR has previously been linked to hyperproduction of anguibactin and was found in all the European isolates. However, overall the pJM1-like plasmids isolated from V. anguillarum serovar O1 exhibited a high degree of conservation regardless of their geographical origin or fish species.
对鳗弧菌pJM1样质粒的遗传多样性进行了研究。从18株来自不同地理位置和鱼类品种的鳗弧菌血清型O1菌株中分离出质粒。对这些质粒进行测序，并与已发表的毒力质粒pJM1的完整序列进行比较。所有18株菌株都含有约65 kbp的pJM1样质粒，且所有质粒都编码负责anguibactin铁螯合系统的毒力基因。这些质粒高度保守，但在一些基因中观察到细微差异。单核苷酸多态性（SNP）分析显示，18个质粒中的每一个与pJM1质粒之间存在0至11个核苷酸变异。与pJM1的序列相比，在fatC、angR、angL、pJM1_p19和angE中鉴定出非同义SNP。特别是，在18个测序质粒中的15个中发现的angR突变先前已与anguibactin的过量产生有关，并且在所有欧洲分离株中都发现了该突变。然而，总体而言，从鳗弧菌血清型O1分离出的pJM1样质粒无论其地理来源或鱼类品种如何，都表现出高度的保守性。
