用于生物催化合成2,5-呋喃二甲酸的工程化BF60的固定化。
Immobilization of the engineered BF60 for biocatalytic synthesis of 2, 5-furandicarboxylic acid.
作者信息
Zhang Boyue, Yuan Haibo, Li Jianghua, Liu Long, Peng Xiaojun
机构信息
State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Hi-tech Zone, Dalian 116024, China.
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, China.
出版信息
J Microbiol Biotechnol. 2020 Jan 9. doi: 10.4014/jmb.1911.11044.
As a bio-based platform chemical, 2, 5-furandicarboxylic acid (FDCA) is a promising substitute of terephthalic acid to synthesize poly (ethylene furanoate) used for bio-substitute of polyethylene terephthalate (PET). In previous work, we engineered a BF60 strain and achieved the effective biocatalytic synthesis of FDCA from 5-hydroxymethylfurfural (HMF). Herein, to improve the reusability of the engineered BF60 cells, we used two materials PVA1799-boric acid and sodium alginate-Ca to immobilize the cells. The sodium alginate-Ca was more suitable for FDCA production, and 22 mmol/L FDCA was obtained with Ca(HPO) as the cross-linker. In addition, the immobilization conditions were optimized and FDCA production was further increased to 74.8 mmol/L. Here the developed immobilization strategy for BF60 cells may be helpful for the economical production of FDCA in the future.
作为一种生物基平台化学品，2,5-呋喃二甲酸（FDCA）是合成聚（呋喃二甲酸乙二酯）以用于聚对苯二甲酸乙二酯（PET）生物替代品的对苯二甲酸的一种有前景的替代品。在之前的工作中，我们构建了BF60菌株，并实现了由5-羟甲基糠醛（HMF）有效生物催化合成FDCA。在此，为提高工程化BF60细胞的可重复使用性，我们使用了两种材料聚乙烯醇1799-硼酸和海藻酸钠-钙来固定细胞。海藻酸钠-钙更适合用于FDCA生产，以磷酸氢钙（Ca(HPO)）作为交联剂时可获得22 mmol/L的FDCA。此外，对固定化条件进行了优化，FDCA产量进一步提高到74.8 mmol/L。此处所开发的BF60细胞固定化策略可能有助于未来FDCA的经济生产。
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