在恶臭假单胞菌 BF60 中过表达 5-羟甲基糠醛氧化酶和 5-羟甲基糠醛/糠醛氧化还原酶提高 2,5-呋喃二甲酸的产量。
Improved production of 2,5-furandicarboxylic acid by overexpression of 5-hydroxymethylfurfural oxidase and 5-hydroxymethylfurfural/furfural oxidoreductase in Raoultella ornithinolytica BF60.
机构信息
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA.
出版信息
Bioresour Technol. 2018 Jan;247:1184-1188. doi: 10.1016/j.biortech.2017.08.166. Epub 2017 Sep 1.
2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block and can be produced by biotransformation of 5-hydroxymethylfurfural (HMF). To improve the FDCA production, two genes-one encoding HMF oxidase (HMFO; from Methylovorus sp. strain MP688) and another encoding for HMF/Furfural oxidoreductase (HmfH; from Cupriavidus basilensis HMF14)-were introduced into Raoultella ornithinolytica BF60. The FDCA production in the engineered whole-cell biocatalyst increased from 51.0 to 93.6mM, and the molar conversion ratio of HMF to FDCA increased from 51.0 to 93.6%.
2,5-呋喃二甲酸（FDCA）是一种很有前途的生物基建筑模块，可以通过 5-羟甲基糠醛（HMF）的生物转化来生产。为了提高 FDCA 的产量，我们将两个基因（一个编码 HMF 氧化酶（HMFO；来自 Methylovorus sp. strain MP688），另一个编码 HMF/Furfural 氧化还原酶（HmfH；来自 Cupriavidus basilensis HMF14））引入到 Raoultella ornithinolytica BF60 中。在工程化的全细胞生物催化剂中，FDCA 的产量从 51.0mM 增加到 93.6mM，HMF 到 FDCA 的摩尔转化率从 51.0%增加到 93.6%。
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