Laboratorio de Microbiología Molecular, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Avenida Universidad 3000, colonia UNAM CU, Coyoacán C.P 04510, CdMx, México.
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
J Microbiol Biotechnol. 2020 Apr 28;30(4):497-504. doi: 10.4014/jmb.1908.08056.
For control of brucellosis in small ruminants, attenuated Rev1 is used but it can be virulent for animals and human. Based on these aspects, it is essential to identify potential immunogens to avoid these problems in prevention of brucellosis. The majority of OMPs in the Omp25/31 family have been studied because these proteins are relevant in maintaining the integrity of the outer membrane but their implication in the virulence of the different species of this genus is not clearly described. Therefore, in this work we studied the role of Omp31 on virulence by determining the residual virulence and detecting lesions in spleen and testis of mice inoculated with the LVM31 mutant strain. In addition, we evaluated the conferred protection in mice immunized with the mutant strain against the challenge with the Bm133 virulent strain. Our results showed that the mutation of 31 caused a decrease in splenic colonization without generating apparent lesions or histopathological changes apparent in both organs in comparison with the control strains and that the mutant strain conferred similar protection as the Rev1 vaccine strain against the challenge with Bm133 virulent strain. These results allow us to conclude that Omp31 plays an important role on the virulence of in the murine model, and due to the attenuation shown by the strain, it could be considered a vaccine candidate for the prevention of goat brucellosis.
为了控制小反刍动物中的布鲁氏菌病，使用了减毒 Rev1，但它可能对动物和人类具有毒性。基于这些方面，确定潜在的免疫原以避免在预防布鲁氏菌病中出现这些问题至关重要。Omp25/31 家族中的大多数 OMP 已被研究过，因为这些蛋白质与维持外膜完整性有关，但它们在该属不同种的毒力中的作用尚未清楚描述。因此，在这项工作中，我们通过确定残余毒力并检测接种 LVM31 突变株的小鼠脾脏和睾丸中的病变，研究了 Omp31 在毒力方面的作用。此外，我们评估了用突变株免疫的小鼠对 Bm133 强毒株攻击的保护作用。我们的结果表明，与对照菌株相比，31 突变导致脾脏定植减少，而两个器官均未产生明显的病变或组织病理学变化，并且突变株对 Bm133 强毒株的攻击提供了与 Rev1 疫苗株相似的保护作用。这些结果使我们得出结论，Omp31 在 在小鼠模型中的毒力中起重要作用，并且由于该菌株表现出的衰减，它可以被认为是预防山羊布鲁氏菌病的候选疫苗。
