Golshani Maryam, Rafati Sima, Siadat Seyed Davar, Nejati-Moheimani Mehdi, Shahcheraghi Fereshteh, Arsang Amin, Bouzari Saeid
Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Department of Molecular Immunology and Vaccine Research, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran.
Mol Immunol. 2015 Aug;66(2):384-91. doi: 10.1016/j.molimm.2015.04.015. Epub 2015 May 18.
Brucellosis is one of the most common zoonotic diseases caused by species of Brucella. At present, there is no commercially available vaccine for the human brucellosis. Brucella melitensis and Brucella abortus are the main causes of human brucellosis, worldwide. The outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved among human Brucella pathogens. The purpose of the current study was to evaluate and compare the immunogenicity and protective efficacy of the L7/L12-TOmp31 construct administered as DNA/DNA and DNA/Pro vaccine regimens. Vaccination of BALB/c mice with the DNA/Pro regimen provided more protection levels against B. melitenisis and B. abortus challenge than did the DNA/DNA regimen. IgG1 and IgG2a titers were higher in the sera from DNA/Pro-immunized mice than in those from mice immunized with DNA alone. Moreover, splenocytes from DNA/Pro-immunized mice produced significantly higher levels of IFN-γ than did those from mice given DNA alone. The pcDNA-L7/L12-TOmp31 priming followed by rL7/L12-TOmp31 boosting led to improved protection against B. abortus or B. melitensis infection.
布鲁氏菌病是由布鲁氏菌属引起的最常见人畜共患病之一。目前，尚无用于人类布鲁氏菌病的市售疫苗。在全球范围内，羊种布鲁氏菌和牛种布鲁氏菌是人类布鲁氏菌病的主要病因。外膜蛋白31（Omp31）和L7/L12是人类布鲁氏菌病原体中保守的免疫显性和保护性抗原。本研究的目的是评估和比较以DNA/DNA和DNA/Pro疫苗方案接种的L7/L12-TOmp31构建体的免疫原性和保护效力。与DNA/DNA方案相比，用DNA/Pro方案给BALB/c小鼠接种疫苗对羊种布鲁氏菌和牛种布鲁氏菌攻击提供了更高的保护水平。DNA/Pro免疫小鼠血清中的IgG1和IgG2a滴度高于仅用DNA免疫的小鼠。此外，DNA/Pro免疫小鼠的脾细胞产生的IFN-γ水平明显高于仅给予DNA的小鼠。先用pcDNA-L7/L12-TOmp31进行初免，然后用rL7/L12-TOmp31进行加强免疫，可提高对牛种布鲁氏菌或羊种布鲁氏菌感染的保护作用。
