Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Curr Drug Deliv. 2020;17(2):174-183. doi: 10.2174/1567201817666200120120105.
An injectable long acting In-Situ Forming Gel (ISFG) of human Growth Hormone (hGH) was prepared by using triblock PCL-PEG-PCL (Mw 1500-1500-1500). Ring-Opening Polymerization (ROP) of triblock using microwave was applied.
The BCA protein assay Kit was used to determine the concentration of hGH in the in-vitro release medium. Finally, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) tests and Circular Dichroism (CD) spectrum were done to approve the stability of released hGH. The result of ROP demonstrated that the proportion of PCL to PEG accorded with the initial molar ratio of the monomers. The cross-section of the Surface Electron Microscopy (SEM) indicated the porous framework of the hydrogel could load the drug into its tridimensional matrixes structure. There is the low initial burst release of hGH from the supramolecular hydrogel.
The maximum in-vitro release of hGH was 71.2 % ± 1.5 that were due to hGH degrading after this time (21 days). The CD spectrum and SDS-PAGE results confirmed the stability of hGH during invitro release evaluation.
The results suggest that the sustained-release formulation using PCL-PEG-PCL can be applied to control the release of hGH.
通过使用三嵌段聚己内酯-聚乙二醇-聚己内酯（MW1500-1500-1500）制备了可注射长效原位形成凝胶（ISFG）的人生长激素（hGH）。应用微波进行三嵌段开环聚合（ROP）。
使用 BCA 蛋白测定试剂盒测定体外释放介质中 hGH 的浓度。最后，进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测试和圆二色性（CD）光谱测试，以验证释放的 hGH 的稳定性。ROP 的结果表明，PCL 与 PEG 的比例与单体的初始摩尔比相符。表面电子显微镜（SEM）的横截面表明，水凝胶的多孔骨架可以将药物负载到其三维基质结构中。超分子水凝胶中 hGH 的初始突释较低。
hGH 的最大体外释放量为 71.2%±1.5%，这是由于 hGH 在 21 天后降解所致。CD 光谱和 SDS-PAGE 结果证实了 hGH 在体外释放评价过程中的稳定性。
结果表明，使用 PCL-PEG-PCL 的缓释制剂可用于控制 hGH 的释放。
