Chronic Disease Research Center, Medical College, Dalian University, Dalian 116622, Liaoning, China.
Biomedical Translational Research Institute, Jinan University, Guangdong 510632, Guangzhou, China.
Life Sci. 2020 Mar 15;245:117354. doi: 10.1016/j.lfs.2020.117354. Epub 2020 Jan 25.
Hepatitis B X-interacting protein (HBXIP) is highly expressed in many cancers, but the correlation between the expression of HBXIP and the clinical significance and underlying molecular mechanisms in colorectal cancer (CRC) is still unclear. We selected 186 specimens from CRC patients for analyzing the relationship between the expression of HBXIP and the clinical-pathological features by immunohistochemistry. Migration and invasion experiments were performed to examine the effect of HBXIP on CRC cell metastasis. Besides, we also explored the possible molecular mechanism of HBXIP regulation of CRC cell metastasis by Western blot. Our data indicated that the HBXIP was overexpressed in CRC tissues. High HBXIP expression was correlated with metastasis and shorter survival times in patients with CRC and served as an independent factor for poor prognosis. Moreover, HBXIP promotes CRC metastasis by enhancing the epithelial-mesenchymal transition (EMT) process. Our findings provide the first evidence that HBXIP induces EMT to promote metastasis and predicts the poor prognosis of CRC. Therefore, HBXIP may become a new target for CRC treatment.
乙型肝炎病毒 X 相互作用蛋白 (HBXIP) 在许多癌症中高度表达，但 HBXIP 的表达与结直肠癌 (CRC) 的临床意义和潜在分子机制之间的相关性尚不清楚。我们选择了 186 例 CRC 患者的标本，通过免疫组织化学分析 HBXIP 的表达与临床病理特征之间的关系。通过迁移和侵袭实验研究 HBXIP 对 CRC 细胞转移的影响。此外，我们还通过 Western blot 探讨了 HBXIP 调节 CRC 细胞转移的可能分子机制。我们的数据表明，HBXIP 在 CRC 组织中过度表达。高 HBXIP 表达与 CRC 患者的转移和较短的生存时间相关，并作为预后不良的独立因素。此外，HBXIP 通过增强上皮-间充质转化 (EMT) 过程促进 CRC 转移。我们的研究结果首次提供了 HBXIP 通过诱导 EMT 促进转移并预测 CRC 不良预后的证据。因此，HBXIP 可能成为 CRC 治疗的新靶点。
