Pacey Evan K, Maherali Hafiz, Husband Brian C
Department of Integrative Biology University of Guelph Guelph ON Canada.
Ecol Evol. 2019 Dec 18;10(1):198-216. doi: 10.1002/ece3.5886. eCollection 2020 Jan.
Whole genome duplication, leading to polyploidy and endopolyploidy, occurs in all domains and kingdoms and is especially prevalent in vascular plants. Both polyploidy and endopolyploidy increase cell size, but it is unclear whether both processes have similar effects on plant morphology and function, or whether polyploidy influences the magnitude of endopolyploidy. To address these gaps in knowledge, fifty-five geographically separated diploid accessions of that span a gradient of endopolyploidy were experimentally manipulated to induce polyploidy. Both the diploids and artificially induced tetraploids were grown in a common greenhouse environment and evaluated with respect to nine reproductive and vegetative characteristics. Induced polyploidy decreased leaf endopolyploidy and stem endopolyploidy along with specific leaf area and stem height, but increased days to bolting, leaf size, leaf dry mass, and leaf water content. Phenotypic responses to induced polyploidy varied significantly among accessions but this did not affect the relationship between phenotypic traits and endopolyploidy. Our results provide experimental support for a trade-off between induced polyploidy and endopolyploidy, which caused induced polyploids to have lower endopolyploidy than diploids. Though polyploidy did not influence the relationship between endopolyploidy and plant traits, phenotypic responses to experimental genome duplication could not be easily predicted because of strong cytotype by accession interactions.
全基因组复制会导致多倍体和核内多倍体的产生,它在所有的域和界中都会发生,在维管植物中尤为普遍。多倍体和核内多倍体都会增加细胞大小,但尚不清楚这两个过程对植物形态和功能的影响是否相似,或者多倍体是否会影响核内多倍体的程度。为了填补这些知识空白,对55个地理上分离的二倍体种质进行了实验操作,这些种质跨越了核内多倍体的梯度,以诱导多倍体的产生。二倍体和人工诱导的四倍体都在一个共同的温室环境中生长,并对九个生殖和营养特征进行了评估。诱导多倍体降低了叶片和茎的核内多倍体以及比叶面积和茎高,但增加了抽薹天数、叶片大小、叶片干质量和叶片含水量。不同种质对诱导多倍体的表型反应差异显著,但这并不影响表型性状与核内多倍体之间的关系。我们的结果为诱导多倍体和核内多倍体之间的权衡提供了实验支持,这导致诱导多倍体的核内多倍体低于二倍体。虽然多倍体没有影响核内多倍体与植物性状之间的关系,但由于种质与细胞型之间的强烈相互作用,实验性基因组复制的表型反应不易预测。