Department of Dermatology, The Second Affiliated Hospital, Anhui Medical University, Hefei 23000, Anhui, PR China.
The Institute of Plasma Physics, Chinese Academy of Science, Hefei 230000, Anhui, PR China.
Future Microbiol. 2020 Jan;15:115-125. doi: 10.2217/fmb-2019-0265. Epub 2020 Jan 28.
The global of spread multidrug-resistant has become a public health threat. Cold atmospheric plasma (CAP) is reported to have bactericidal efficacy; however, its effects on clinical super multidrug-resistant are unclear. The aim of this study was to investigate the bactericidal efficacy of CAP on a strain of super multidrug-resistant . The effects of CAP treatments were evaluated using assays for the detection of growth, viability, metabolism, virulence factors and reactive oxygen species (ROS) levels. Both CAP treatments dose-dependently inhibited cell viability and metabolic activity, and decreased the expression of several virulence factors. CAP treatment induced a significant increase in intracellular ROS levels, and ROS scavengers inhibited this effect. CAP treatment is a promising option for the clinical inhibition of multidrug-resistant , and the development of CAP technologies might be the key to solving the long-standing problem of drug-resistant bacteria.
全球蔓延的多药耐药性已经成为公共卫生威胁。据报道，冷等离体等离子体（CAP）具有杀菌功效；然而，其对临床超级多重耐药的影响尚不清楚。本研究的目的是研究 CAP 对超级多重耐药株的杀菌效果。通过检测生长、活力、代谢、毒力因子和活性氧（ROS）水平的测定来评估 CAP 处理的效果。CAP 处理均呈剂量依赖性地抑制细胞活力和代谢活性，并降低了几种毒力因子的表达。CAP 处理诱导细胞内 ROS 水平显著增加，而 ROS 清除剂抑制了这种作用。CAP 处理是临床抑制多药耐药的有前途的选择，而 CAP 技术的发展可能是解决耐药菌这一长期问题的关键。
