Xu Dehui, Wang Shuai, Li Bing, Qi Miao, Feng Rui, Li Qiaosong, Zhang Hao, Chen Hailan, Kong Michael G
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an 710049, China.
The school of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Microorganisms. 2020 Jul 21;8(7):1091. doi: 10.3390/microorganisms8071091.
Cold atmospheric plasma (CAP) has been widely used in biomedicine during the last two decades. While direct plasma treatment has been reported to promote wound healing, its application can be uneven and inconvenient. In this study, we first activated water with a portable dielectric barrier discharge plasma device and evaluated the inactivation effect of plasma-activated water (PAW) on several kinds of bacteria that commonly infect wounds. The results show that PAW can effectively inactivate these bacteria. Then, we activated tap water and examined the efficacy of PAW on wound healing in a mouse model of full-thickness skin wounds. We found that wound healing in mice treated with PAW was significantly faster compared with the control group. Histological analysis of the skin tissue of mice wounds showed a significant reduction in the number of inflammatory cells in the PAW treatment group. To identify the possible mechanism by which PAW promotes wound healing, we analyzed changes in the profiles of wound bacteria after PAW treatment. The results show that PAW can significantly reduce the abundance of wound bacteria in the treatment group. The results of biochemical blood tests and histological analysis of major internal organs in the mice show that PAW had no obvious side effects. Taken together, these results indicate that PAW may be a new and effective method for promoting wound healing without side effects.
在过去二十年中，低温大气等离子体（CAP）已在生物医学中得到广泛应用。虽然据报道直接等离子体处理可促进伤口愈合，但其应用可能不均匀且不方便。在本研究中，我们首先使用便携式介质阻挡放电等离子体装置活化水，并评估了等离子体活化水（PAW）对几种常见伤口感染细菌的灭活效果。结果表明，PAW可有效灭活这些细菌。然后，我们活化自来水，并在全层皮肤伤口小鼠模型中研究了PAW对伤口愈合的疗效。我们发现，与对照组相比，用PAW处理的小鼠伤口愈合明显更快。对小鼠伤口皮肤组织的组织学分析表明，PAW治疗组的炎症细胞数量显著减少。为了确定PAW促进伤口愈合的可能机制，我们分析了PAW处理后伤口细菌谱的变化。结果表明，PAW可显著降低治疗组伤口细菌的丰度。对小鼠主要内脏器官的生化血液检测和组织学分析结果表明，PAW没有明显的副作用。综上所述，这些结果表明，PAW可能是一种促进伤口愈合且无副作用的新的有效方法。
