Universidade Federal de Pelotas (UFPel), Campus Universitário, S/N, Caixa Postal 354, CEP 96010-900, Pelotas, RS, Brazil.
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Pecuária Sul, Sanidade Animal, Bage, RS, Brazil.
Benef Microbes. 2020 Mar 27;11(2):175-181. doi: 10.3920/BM2019.0120. Epub 2020 Jan 28.
Studies aiming at the development and evaluation of alternative methods to minimise losses caused by the gastrointestinal nematode are extremely important. Such research is essential, given the high morbidity rates among sheep and the significant mortality rates of lambs, allied to the low efficacy of commercial products for the control of this parasite. The purpose of this study was to evaluate the effect of the (YT001 - YEASTECH) on the control of and its modulation of the immune response in experimentally infected sheep. Eighteen sheep were divided into two groups. Group 1, the control group, comprised animals infected with and supplemented with distilled water, while Group 2, the treated group, consisted of animals infected and supplemented with (400 million cfu/day of suspension for 49 days). The following parasitological parameters were evaluated: number of eggs per gram of faeces, number of infective larvae (L3) recovered per faecal culture, and parasitic load of the abomasum. The following immunological parameters were quantified: immunoglobulin (Ig)A in the mucous secretions and serum IgG; cytokines interleukin (IL)-4, IL-5 and IL-10; number of eosinophils in the abomasal mucosa and groups of cells positive for the markers: MHCII, CD4CD25, CD5CD8, WC4, CD5CD4, CD8CD11b and CD5WC1 by whole blood flow cytometry. The results revealed a significant decrease (<0.05) in the number of larvae and significantly higher serum IgG levels (<0.05) in the group supplemented with . The supplemented animals showed significantly larger numbers of eosinophils (<0.05), as well as more cells positive for MHCII, CD4CD25, CD5CD8 than the control animals. This study confirmed the beneficial action of on the host immune response to , as evidenced mainly by the smaller number of L3 recovered from the faeces of sheep supplemented with .
研究旨在开发和评估替代方法，以尽量减少胃肠道线虫造成的损失，这是非常重要的。鉴于绵羊的高发病率和羔羊的高死亡率，以及商业产品对这种寄生虫控制的低效果，这种研究是必不可少的。本研究的目的是评估 （YT001-YEASTECH）对 的控制及其对实验感染绵羊免疫反应的调节作用。将 18 只绵羊分为两组。第 1 组为对照组，由感染 的绵羊组成，并补充蒸馏水；第 2 组为治疗组，由感染 并补充 （49 天每天 4 亿个 cfu 的混悬液）的绵羊组成。评估了以下寄生虫参数：粪便中每克的卵数、每粪便培养中回收的感染性幼虫（L3）数、和胃的寄生虫负荷。量化了以下免疫参数：黏膜分泌物和血清 IgG 的免疫球蛋白（Ig）A；细胞因子白细胞介素（IL）-4、IL-5 和 IL-10；胃黏膜中的嗜酸性粒细胞数和标记物阳性的细胞群：MHCII、CD4CD25、CD5CD8、WC4、CD5CD4、CD8CD11b 和 CD5WC1 通过全血流式细胞术。结果显示，补充 的组幼虫数量显著减少（<0.05），血清 IgG 水平显著升高（<0.05）。补充动物的嗜酸性粒细胞数量明显增加（<0.05），并且 MHCII、CD4CD25、CD5CD8 阳性的细胞数也多于对照组动物。这项研究证实了 对宿主对 的免疫反应的有益作用，主要证据是从补充 的绵羊粪便中回收的 L3 数量减少。
