Department of Parasitology, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brazil.
Faculdade de Ciências Médicas, Universidade de Pernambuco, Recife, Brazil.
Parasite Immunol. 2020 Apr;42(4):e12701. doi: 10.1111/pim.12701.
The aim of the present study was to assess serum cytokine and miRNA expression in visceral leishmaniasis-HIV (VL-HIV) co-infection and HIV mono-infection.
We analysed 113 serum samples from HIV patients in areas endemic for leishmaniasis. The diagnosis of VL was confirmed in 65 of these 113 samples. The VL-HIV and HIV groups presented significant differences regarding haemoglobin level (P < .0001), lymphocyte count (P = .0444), white blood cell count (P = .0108), weight loss (P = .0310), HIV load (P < .0001) and CD4+ T-lymphocytes count (P = .0003). Levels of IL-6 and IL-10, IFN-γ and IL-6, IFN-γ and IL-10, TNF and IL-2 were positively correlated in VL-HIV co-infection, indicating higher serum levels of TNF and IL-4 (P < .0001). In addition, miR-182 expression was found to be significantly higher in HIV (P = .009), miR-210 exhibited no statistically significant difference between groups, and nonexpression of miR-122 was found in both groups.
Together, TNF, IL-4 and miR-182 may represent circulatory biomarkers of VL-HIV co-infection.
本研究旨在评估内脏利什曼病-人类免疫缺陷病毒（VL-HIV）合并感染和人类免疫缺陷病毒单一感染患者血清细胞因子和 miRNA 表达。
我们分析了来自利什曼病流行地区的 113 例 HIV 患者的血清样本。在这 113 个样本中，有 65 个被确诊为 VL。VL-HIV 组和 HIV 组在血红蛋白水平（P<.0001）、淋巴细胞计数（P=.0444）、白细胞计数（P=.0108）、体重减轻（P=.0310）、HIV 载量（P<.0001）和 CD4+T 淋巴细胞计数（P=.0003）方面存在显著差异。IL-6 和 IL-10、IFN-γ 和 IL-6、IFN-γ 和 IL-10、TNF 和 IL-2 在 VL-HIV 合并感染中呈正相关，表明 TNF 和 IL-4 的血清水平更高（P<.0001）。此外，miR-182 在 HIV 组中的表达明显更高（P=.009），miR-210 在两组之间无统计学差异，两组均未检出 miR-122。
综上所述，TNF、IL-4 和 miR-182 可能是 VL-HIV 合并感染的循环生物标志物。
