Satou N, Satou J, Shintani H, Okuda K
Department of Operative Dentistry, Hiroshima University, School of Dentistry, Japan.
J Gen Microbiol. 1988 May;134(5):1299-305. doi: 10.1099/00221287-134-5-1299.
Four types of surface-modified glass were prepared. Aminopropyl glass was prepared by alkylsilylation of glass slides with gamma-aminopropyltriethoxysilane. This glass carries primary amino groups which may be protonated at pH 7.2. Owing to the presence of both positively charged ions and hydrophobic ethoxyl groups, the glass is considered to be amphipathic. Three other types of surface-modified glass slides were prepared from aminopropyl glass by forming Schiff's bases with three aldehydes: glucose, glyoxylic acid and hexanal. The aldehyde-treated slides were subsequently reduced using sodium borohydride. Thus, the surface of the glass was rendered hydrophilic, ampholytic or hydrophobic, respectively. The adherence of two Streptococcus sanguis strains and two Streptococcus mutans strains to the surface-modified glass slides was studied. Different strains showed differences in adherence to these slides depending on their physico-chemical surface properties. For S. sanguis ATCC 10556, hydrophobic bonds seemed to be most important, while in S. mutans OMZ 176, ionic interactions made the highest contribution to adhesion. Hydrogen bonds seemed to contribute least to adherence.
制备了四种表面改性玻璃。氨基丙基玻璃是通过用γ-氨基丙基三乙氧基硅烷对载玻片进行烷基硅烷化制备的。这种玻璃带有伯氨基，在pH 7.2时可能会质子化。由于存在带正电荷的离子和疏水性乙氧基，这种玻璃被认为是两亲性的。通过用三种醛（葡萄糖、乙醛酸和己醛）形成席夫碱，由氨基丙基玻璃制备了另外三种类型的表面改性载玻片。随后用硼氢化钠还原经醛处理的载玻片。因此，玻璃表面分别变得亲水、两性或疏水。研究了两种血链球菌菌株和两种变形链球菌菌株对表面改性载玻片的粘附情况。不同菌株对这些载玻片的粘附表现出差异，这取决于它们的物理化学表面性质。对于血链球菌ATCC 10556，疏水键似乎最为重要，而在变形链球菌OMZ 176中，离子相互作用对粘附的贡献最大。氢键对粘附的贡献似乎最小。
