Cowan M M, van der Mei H C, Rouxhet P G, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
J Gen Microbiol. 1992 Dec;138(12):2707-14. doi: 10.1099/00221287-138-12-2707.
The surface properties of nine Streptococcus mitis and four Peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. Zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. In addition, elemental surface concentration ratios were obtained via X-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. The S. mitis and P. micros strains were found to have higher isoelectric points, higher hydrophobicities and higher N/C surface concentration ratios than some other oral streptococci. The combined data suggest that both species possess large amounts of surface protein. All the S. mitis strains displayed abundant surface fibrils in negative staining, but the P. micros strains were devoid of surface appendages indicating that surface protein is present in different forms in the two species. The surfaces of S. mitis and P. micros type strains differed significantly from the other strains examined.
对来自口腔的9株缓症链球菌和4株微小消化链球菌菌株的表面特性进行了检测，并与一大组口腔链球菌进行了比较。采用zeta电位和接触角测量来确定细胞表面的物理化学性质。此外，通过X射线光电子能谱获得元素表面浓度比，并用透射电子显微镜检查表面结构。发现缓症链球菌和微小消化链球菌菌株比其他一些口腔链球菌具有更高的等电点、更高的疏水性和更高的N/C表面浓度比。综合数据表明，这两个菌种都含有大量的表面蛋白。所有缓症链球菌菌株在负染色中均显示出丰富的表面纤维，但微小消化链球菌菌株没有表面附属物，这表明表面蛋白在这两个菌种中以不同形式存在。缓症链球菌和微小消化链球菌模式菌株的表面与所检测的其他菌株有显著差异。
