Department of Biochemistry, College of Science, Evangel University Akaeze, P.M.B. 129 Abakaliki, Ebonyi State, Nigeria.
Department of Biochemistry, College of Medical Sciences, University of Calabar, P.M.B 1115, Calabar, Cross River State, Nigeria.
Biomed Pharmacother. 2020 Apr;124:109879. doi: 10.1016/j.biopha.2020.109879. Epub 2020 Jan 25.
African walnuts were previously shown to modulate hepatic lipid bio-accumulation in obesity. Herein, we investigated the impact of the nuts on fat accumulation in adipose and ectopic regions, and associated oxidatiive stress status in obese rats.
Whole ethanol extract (WE) of the nuts, and its liquid-liquid fractions-ethyl acetate (ET) and residue (RES) were separately administered to obese rats for 6 weeks. The normal (NC) and obese (OC) controls received normal saline and the standard control (SC), orlistat (5.14 mg/kg b.w.), during the same period. Thereafter, the animals were euthanized and the adipose, brain, kidneys and heart tissues were studied.
The change in body weight to naso-anal length which increased by 63.52 % in OC compared to NC (p < 0.05), decreased by 57.88, 85.80 and 70.20 % in WE, ET and RES-treated groups, respectively, relative to the OC (p < 0.05). Also, adipose tissue weights were lowered upon treatment with the extracts and fractions versus OC (p < 0.05). Total lipids, phospholipids, triacylglycerol and cholesterol concentrations in the studied tissues which were higher in OC (p < 0.05) were lowered (p < 0.05) and compared favorably with SC. Further, malondialdehyde levels in the tissues were lowered upon treatment, compared to the OC (p < 0.05). Glutathione level and activities of glutathione peroxidase, superoxide dismutase and glutathione-S-transferase which were decreased (p < 0.05) in OC, were restored upon treatment with the extracts, relative to the obese control (p < 0.05).
African walnuts assuaged lipogenesis, oxidative stress and peroxidation in extra-hepatic tissues of obese rats, hence, may attenuate ectopic fat accumulation and its associated pathogenesis.
先前的研究表明，非洲核桃可调节肥胖症患者的肝脏脂质生物蓄积。在此，我们研究了这些坚果对肥胖大鼠脂肪在脂肪组织和异位组织中积累的影响，以及与氧化应激状态相关的影响。
将坚果的全乙醇提取物（WE）及其液-液部分-乙酸乙酯（ET）和残渣（RES）分别施用于肥胖大鼠 6 周。正常（NC）和肥胖（OC）对照组在同一时期接受生理盐水和标准对照（SC）或奥利司他（5.14mg/kg b.w.）。之后，处死动物，研究其脂肪组织、大脑、肾脏和心脏组织。
OC 组的体重到鼻肛长的变化比 NC 组增加了 63.52%（p<0.05），而 WE、ET 和 RES 治疗组的体重变化分别降低了 57.88%、85.80%和 70.20%，相对于 OC 组（p<0.05）。此外，与 OC 组相比，各提取物和馏分治疗组的脂肪组织重量降低（p<0.05）。研究组织中的总脂质、磷脂、三酰甘油和胆固醇浓度在 OC 组中较高（p<0.05），经治疗后降低（p<0.05），与 SC 相比具有优势。进一步的，与 OC 组相比，各治疗组的组织中丙二醛水平降低（p<0.05）。OC 组中谷胱甘肽水平和谷胱甘肽过氧化物酶、超氧化物歧化酶和谷胱甘肽-S-转移酶的活性降低（p<0.05），经提取物治疗后恢复，相对于肥胖对照组（p<0.05）。
非洲核桃减轻了肥胖大鼠肝外组织中的脂肪生成、氧化应激和过氧化作用，因此，可能减轻异位脂肪积累及其相关的发病机制。
