Department of Nutrition, Master of Biomedical Nutrition Program, HungKuang University, Taichung43302, Taiwan.
Department of Internal Medicine, Kuang-Tien General Hospital, Taichung43302, Taiwan.
Nutrients. 2020 Jan 26;12(2):321. doi: 10.3390/nu12020321.
Chronic hepatitis B virus (HBV) infection is a serious public health issue. Vitamin D is involved in various pathophysiological mechanisms as an immune modulator and the deficiency rate of vitamin D is prevalent in chronic liver disease. Fucoidan exerts anti-inflammatory, anticoagulant, antitumor, antimetastatic, and antiangiogenetic effects; however, its effect on the immune responses of HBV patients is unclear. This study investigated how 25(OH)Vitamin D status affected the effectiveness of oligo fucoidan in patients with HBV infection in the immune tolerance phase. Fifty-one patients received oligo fucoidan 4400 mg/day for 48 weeks. Flow cytometry was used to detect T lymphocyte markers (CD3CD4, CD3CD8, CD4CD45RO, CD8CD45RO). The levels of white blood cell (WBC), platelets (PLT), and albumin were decreased after 48 weeks of supplementation ( 0.05). Percentages of CD3CD8 and CD8CD45RO+ cells were decreased after 12 weeks of supplementation ( 0.05). In patients with adequate vitamin D, HBV-DNA concentrations decreased and the proportion of CD4CD45RO and CD8CD45RO cells increased upon oligo fucoidan supplementation. The HBeAg status of one vitamin D-adequate patient changed from positive to negative at the 12th week of supplementation. The oligo fucoidan may regulate immune effects in patients with HBV infection, and the 25(OH)Vitamin D status might have affected the effectiveness of oligo fucoidan.
慢性乙型肝炎病毒 (HBV) 感染是一个严重的公共卫生问题。维生素 D 作为一种免疫调节剂参与多种病理生理机制，其在慢性肝病中的缺乏率很普遍。褐藻糖胶具有抗炎、抗凝、抗肿瘤、抗转移和抗血管生成作用；然而，其对 HBV 患者免疫反应的影响尚不清楚。本研究探讨了 25(OH)维生素 D 状态如何影响免疫耐受期 HBV 感染患者寡聚褐藻糖胶的疗效。51 例患者每天接受寡聚褐藻糖胶 4400mg 治疗 48 周。采用流式细胞术检测 T 淋巴细胞标志物（CD3CD4、CD3CD8、CD4CD45RO、CD8CD45RO）。补充 48 周后，白细胞（WBC）、血小板（PLT）和白蛋白水平降低（ 0.05）。补充 12 周后，CD3CD8 和 CD8CD45RO+细胞的百分比降低（ 0.05）。在维生素 D 充足的患者中，HBV-DNA 浓度降低，寡聚褐藻糖胶补充后 CD4CD45RO 和 CD8CD45RO 细胞的比例增加。1 例维生素 D 充足的患者的 HBeAg 状态在补充 12 周时从阳性变为阴性。寡聚褐藻糖胶可能调节 HBV 感染患者的免疫作用，25(OH)维生素 D 状态可能影响寡聚褐藻糖胶的疗效。
