Douthit Christian, Gudenkauf Brent, Hamood Abdul, Mudaliar Nithya, Caroom Cyrus, Jenkins Mark
Texas Tech University Health Sciences Center, Lubbock, TX, USA.
J Clin Orthop Trauma. 2020 Feb;11(Suppl 1):S113-S117. doi: 10.1016/j.jcot.2019.10.002. Epub 2019 Oct 13.
Hardware infections in orthopedic surgery, specifically those involving biofilm producing bacteria, are troublesome and are highly resistant to systemic antibiotics. The purpose of this study was to demonstrate the power of rifampin and vancomycin solutions in inhibiting as well as eliminating on staphylococcus aureus () biofilm on stainless-steel implants.
A suspension of either or a containing a plasmid that cods for the green fluorescence protein containing fluorescent protein plasmid was applied to 1 × 1cm sterile stainless steel orthopedic plating material (coupon). Biofilm development was confirmed by; the quantitative assay (colony forming unit [CFU/coupon]) and visualized using confocal laser scanning microscopy. With this established method of biofilm development, we determined the minimum biofilm inhibitory concentration (MBIC) and the minimum biofilm eradication concertation (MBEC) of Rifampicin and Vancomycin. To determine the MBIC, stainless steel plates were subjected to different concentrations of antibiotic solution and inoculated with overnight cultures of . After 24 h of incubation at 37 °C, the biofilms on the untreated and antibiotic-treated coupons were quantified. To determine the MBEC, partial biofilms were developed on the coupons and then treated with the different concentrations of each antibiotic for 24 h. The number of bacteria within the control untreated as well as treated coupons was determined.
Both rifampin and vancomycin solutions inhibited biofilm production of on stainless steel mediums; the MBIC for rifampin and vancomycin were 80 ng/mL and 1 μg/mL respectively. The MBEC for Rifampicin was similar to the MBIC. However, the MBEC for Vancomycin was 6 mg/ml.
When applied to orthopedic stainless steel hardware , solutions of rifampin and vancomycin powder separately or in combination can completely prevent and eliminate biofilm produced by .
II.
骨科手术中的硬件感染，特别是那些涉及产生生物膜的细菌的感染，很麻烦且对全身抗生素具有高度抗性。本研究的目的是证明利福平溶液和万古霉素溶液在抑制以及消除不锈钢植入物上的金黄色葡萄球菌生物膜方面的作用。
将金黄色葡萄球菌或含有编码绿色荧光蛋白的质粒的金黄色葡萄球菌悬浮液应用于1×1cm无菌不锈钢骨科钢板材料（试样）。通过定量测定（菌落形成单位[CFU/试样]）确认生物膜形成，并使用共聚焦激光扫描显微镜进行可视化。利用这种已建立的生物膜形成方法，我们确定了利福平和万古霉素的最低生物膜抑制浓度（MBIC）和最低生物膜根除浓度（MBEC）。为了确定MBIC，将不锈钢板置于不同浓度的抗生素溶液中，并用金黄色葡萄球菌过夜培养物接种。在37°C孵育24小时后，对未处理和经抗生素处理的试样上的生物膜进行定量。为了确定MBEC，在试样上形成部分金黄色葡萄球菌生物膜，然后用不同浓度的每种抗生素处理24小时。确定对照未处理以及处理过的试样中的细菌数量。
利福平溶液和万古霉素溶液均抑制不锈钢培养基上金黄色葡萄球菌生物膜的产生；利福平和万古霉素的MBIC分别为80 ng/mL和1μg/mL。利福平的MBEC与MBIC相似。然而，万古霉素的MBEC为6mg/ml。
当应用于骨科不锈钢硬件时，利福平溶液和万古霉素粉末单独或联合使用可完全预防和消除金黄色葡萄球菌产生的生物膜。
II级。
